红鳍东方鲀鱼皮胶原低聚肽螯合钙工艺优化

Optimization of Process of Collagen Oligopeptide Calcium-Chelating of Takifugu rubripes Skin

为提高红鳍东方鲀副产物的利用率和附加值,本文以红鳍东方鲀鱼皮为原料制备胶原低聚肽,以螯合率为指标,通过单因素和响应面优化试验对胶原低聚肽与氯化钙螯合工艺进行优化,并通过紫外扫描和傅里叶红外光谱对最优条件下得到的胶原低聚肽螯合钙的结构特征进行初步研究。结果表明,胶原蛋白低聚肽最佳螯合条件为pH9.18,肽钙质量比为20.55∶1,反应温度为45.3℃,在此条件下,肽钙螯合率为91.04%。通过对胶原蛋白低聚肽和胶原低聚肽螯合钙的紫外吸收和红外光谱分析表明,胶原蛋白低聚肽与钙发生螯合反应,生成胶原低聚肽螯合钙,制备出具有补钙作用的新型产品。

To improve the utilization and added value of the byproducts of the Takifugu rubripes.Skin of the Takifugu rubripe was utilized for preparation of collagen oligopeptide. The conditions of collagen oligopeptide chelating calcium chloride were optimized through single factor experiment and response surface methodology with chelating rate as an index. Structure characteristics of freeze-dried sample of collagen oligopeptide chelated calcium were analyzed by Ultraviolet Spectrum(UV),Flourier Transform Infrared Spectrum(FT-IR).The results showed that the optimum chelating conditions were pH9.18,the ratio of peptide to calcium was 20.55∶1,the reaction temperature was 45.3 ℃,under this conditions,the chelating rate was up to 1.04%. Through the UV absorption and infrared spectroscopy analysis of the collagen oligopeptide and calcium-chelating small peptide,it was showed that the collagen oligopeptide reacted with calcium to produce collagen oligopeptide calcium-chelating,and a new type of product with calcium supplementation was prepared.