鸡骨草多糖的酶法提取工艺优化及其抗氧化活性

Optimization of Enzymatic Extraction of Polysaccharide from Abrus cantoniensis Hance and Its Antioxidant Activity

目的:研究纤维素酶提取鸡骨草有效成分多糖的最佳条件,并探讨其体外抗氧化活性。方法:以鸡骨草多糖得率为响应值,在单因素实验基础上,以液料比、酶解时间、酶添加量、酶解温度为自变量,采用响应面法建立数学模型,筛选最佳提取工艺条件,并采用DPPH·和·OH清除能力体系评价鸡骨草多糖体外抗氧化活性。结果:最佳提取条件为:液料比13∶1 m L/g,纤维素酶酶解时间60 min,酶添加量12.8 mg/m L,酶解温度50℃,pH5.0,在此条件下鸡骨草多糖得率为8.15%,与理论值8.34%相对误差小于5%。酶添加量对多糖得率影响最大,液料比、酶解时间次之,酶解温度影响最小。鸡骨草多糖对DPPH·和·OH清除的半数抑制浓度IC_50分别为1.591、1.926 mg/m L,与维生素C比较,抗氧化活性较弱。结论:鸡骨草多糖纤维素酶酶法提取工艺方便可行,酶解得到的多糖具有较强的体外抗氧化活性。

Objective: This study was aimed to optimize polysaccharides extraction from Abrus cantoniensis Hance and investigate its antioxidant activity in vitro.Methods: The mathematical model was established by response surface method( RSM) based on the results of single factor experiments,using polysaccharides extraction rate as response value and the ratio of water to material,enzymolysis extraction time duration,cellulase concentration and enzymolysis extraction temperature as experimental factors,which were used to screen optimum polysaccharide extraction conditions from Abrus cantoniensis Hance. Antioxidant activity of polysaccharides was detected by DPPH· and ·OH scavenging capacity system.Results: The optimum extraction conditions were as follows: The ratio of water to feedstock was 13∶ 1 m L/g,cellulase enzymolysis extraction time duration was 60 min,the cellulase level was 12.8 mg/m L,enzymolysis extraction temperature was 50 ℃,the p H value was 5.0. Under the optimal conditions,there was a difference of less than 5% between predicted extraction rate 8.34% and experimental extraction rate 8.15%.The polysaccharide yield was most significantly affected by cellulase concentration,followed by water to material ratio,extraction time duration and extraction temperature. IC50 of Abrus cantoniensis Hance polysaccharide eliminating DPPH·and ·OH free radical were 1.591 and 1.926 mg/m L,respectively. Antioxidant activity of sample polysaccharides was weaker than those of vitamin C. Conclusion: The polysaccharide extraction process from Abrus cantoniensis Hance by cellulase enzymolysis approach was obtained,which was convenient and feasible,and the extracted polysaccharides had good antioxidant activity in vitro.