GABA处理对双孢蘑菇活性氧代谢和膜脂过氧化的影响

Effects of GABA Treatment on Activate Oxygen Metabolism and Membrane Lipid Peroxidation of Agaricus bisporus

为探讨γ-氨基丁酸(GABA)处理对双孢蘑菇(Agaricus bisporus)活性氧代谢和膜脂过氧化的影响,采用5 mmol/L GABA浸泡处理双孢蘑菇5 min,然后于4℃条件下贮藏12 d,测定双孢蘑菇颜色变化、相对电导率、膜脂过氧化产物丙二醛(MDA)的含量、超氧阴离子生成速率、H_2O_2的含量以及过氧化氢酶(CAT)、超氧化物歧化酶(SOD)等抗氧化酶活性的变化。结果表明,GABA处理能够显著提高抗氧化酶CAT和SOD的活性,降低双孢蘑菇的相对电导率、超氧阴离子产生速率和减少MDA、过氧化氢的积累,延缓细胞膜透性和脂质过氧化进程,进而较好的保持双孢蘑菇的L*值,降低其褐变指数,因此,GABA可以用作双孢蘑菇的护色保鲜剂。

In order to investigate the effect of γ-aminobutyric acid(GABA) treatment on active oxygen metabolism and membrane lipid peroxidation of Agaricus bisporus,A.bisporus were dipped for 5 min in 5 mmol/L GABA,then stored at 4 ℃ for 12 d.Changes of the browning,membrane permeability,lipid peroxidation,reactive oxygen species,and antioxidant enzymes activity in the mushroom tissue were measured. The results showed that the GABA treatment could significantly increase the activities of SOD and CAT,and reduce the relative leakage rate,the production rate of superoxide anion and the accumulation of the contents of MDA and H2O2.And then,the cell membrane permeability and lipid peroxidation were delayed.Furthermore,the browning of A.bisporus was inhibited with higher L* value and lower browning index.Therefore,GABA could be used as a color preservative for A.bisporus.