不同品种山药皮醇提物的抗氧化性能

Antioxidant Activity of Alcohol Extracts from Different Varieties of Yam Peel

本文以菜山药皮为研究对象,烘干后粉碎过筛,通过不同浓度乙醇溶液对菜山药皮粉浸提回流,测定醇提物中多酚、黄酮、皂苷等活性物质含量及以其对DPPH自由基、羟基自由基、超氧阴离子自由基的清除能力以及对铁离子的还原力,评价其抗氧化能力,从而选择出最佳乙醇浓度。然后对铁棍山药皮、蕲春山药皮、利川山药皮、佛手山药皮进行最佳乙醇浓度的浸提,并分析其活性成分和抗氧化能力差异。结果表明:菜山药皮经70%乙醇提取所得到的醇提物,活性成分和抗氧化能力较高;不同品种山药皮醇提物中活性成分和抗氧化能力差异显著(p <0.05),且不同品种山药皮醇提物中活性成分含量与抗氧化能力之间存在显著相关性(p <0.05);其中佛手山药皮醇提物多酚(2.46 mg/g)、皂苷、黄酮以及其他活性成分总量均显著高于其他四种山药皮醇提物(p <0.05),多酚、皂苷、黄酮、活性成分总量分别为12.52、6.12、21.06 mg/g,对羟基自由基、DPPH自由基的清除率以及铁离子还原力也处于较高水平,而对于超氧阴离子自由基的清除率以及亚油酸氧化的抑制能力,佛手山药皮醇提物虽不是最优的,但也处于较高的水平,因此综合来看,佛手山药皮的抗氧化效果要优于其他3种山药皮。

The vegetable yam peel was taken as the research object.After drying,crushing and sieving,the ethanol solution of different concentration of vegetable yam peel powder were extracted by reflux. Determining the contents of polyphenols,flavonoids and saponins and some active substances in alcohol extraction,and the anti-oxidation ability of DPPH radicals,hydroxyl radicals,superoxide anion and the reducing power of iron ions were evaluated to select the optimal ethanol concentration.Then the best ethanol concentration was extracted from the,tiegun yam peel,Qichun yam peel,Lichuan yam peel and bergamot yam peel,and the difference of its active components and antioxidant capacity was analyzed.The results showed that vegetable yam peel extracts obtained by 70% ethanol alcoholhad higher active ingredient and antioxidant capacity. The different varieties of yam peel ethanol extracts difference of bioactive components and antioxidant capacity were significant( p <0.05),and there had a significant correlation between the active components and the antioxidant capacity of the alcohol extracts from different varieties of yam,the ethanol extract of Dioscorea opposita bergamot yam peel polyphenols,saponins,flavonoids and other active ingredient contents were significantly higher than the other four kinds of Chinese yam peel extracts( p < 0.05),polyphenols,saponins,the total flavonoids and active ingredients were 2.46,12.52,6.12 and 21.06 mg/g. The DPPH radical scavenging rate,the hydroxyl radical scavenging rate,and theferric reducing power all at a high level while the superoxide anion scavenging rate and linoleic acid oxidation inhibition ability,bergamot yam peel extracts was not the optimal,but in the high the level.So the bergamot yam peel would be better than other three kind of of Chinese yam peel in antioxidant property.