西双版纳12种食用香料植物精油抑制炎症因子NO活性研究

Activities of Inhibiting Inflammatory Factor NO of Essential Oils from 12 Edible and Fragrant Plants in Xishuangbanna

为了进一步寻找新型高效抗炎药物,本实验选取西双版纳地区12种香料植物,采用水蒸汽蒸馏法提取精油,以脂多糖(Lipopolysaccharides,LPS)刺激RAW264.7细胞,用MTS试剂盒评估细胞活力、Griess试剂盒评估NO产生水平。通过GC-MS联用技术对具有显著抑制作用的精油进行成分分析。结果表明:蒜香藤精油在浓度为0.004%及0.005%时对细胞活力有显著的抑制作用,马来树胡椒精油在浓度为0.005%时,对细胞活力有显著的抑制作用,其他精油在0.001%~0.005%浓度范围内对细胞活力无显著影响或有一定的促进作用;马来树胡椒、锡兰肉桂、清香木、毛叶丁香罗勒、蒜香藤和水香薷具有显著(p <0.0001)的抑制NO产生作用,且其抑制炎症因子NO活性结果由强至弱为:马来树胡椒>水香薷>锡兰肉桂>清香木>蒜香藤>毛叶丁香罗勒,其中马来树胡椒抑制NO率最强为43.68%,除毛叶丁香罗勒精油外,其余抑制活性均优于阳性对照地塞米松;通过GC-MS对六种精油成分进行分析发现,精油成分以烯烃为主。六种精油抑制炎症因子NO产生作用虽然存在差异,但作为一种新型天然抑制炎症NO的材料在药用及功能食品等方面具有较大的潜力。

To find the new and efficient anti-inflammatory medicine,twelve edible and fragrant plants were collected in Xishuangbanna Tropical Botanical Garden.Essential oils were extracted by steam distillation.For anti-inflammatory activity,the cell viability and nitric oxide( NO) production were determined on lipopolysaccharide( LPS)-stimulated RAW264.7 cells by MTS assay and the Griss reagent system,respectively. For the excellent effects on inflammatory,the compounds of them were identified by gas chromatography-mass spectrometry( GC-MS) analysis. The result showed that Mansoa alliacea inhibited cell viability under 0.004% and 0.005% significantly,Piper arborescens inhibited cell viability under 0.005% slightly. Other essential oils had no effects on cell viability or were beneficial to cell viability.Piper arborescens,Cinnamomum verum,Pistacia weinmannifolia,Ocimum Gratissimum var.suave,Mansoa alliacea,Elsholtzia kachinensis exerted efficient effects on production of NO( p < 0.0001),the activity that inhibited production of NO of the six essential oil changed from stronger to weaker as:P.arborescens > E.kachinensis > C. verum > P. weinmannifolia > M. alliacea > O. Gratissimum var. suave. P. arborescens essential oil displayed the strongest inhibiting activity on production of NO which inhibitory rate reached 43.68%. They exerted efficient effects more than Dex except for O.Gratissimum var.suave.It was found that the main components of this six essential oils were alkene by GC-MS. The inhibitory activity of production of NO among these plant essential oils had difference,but these essential oils as a material that new and natural had large potential in medicine and functional foods.