响应面优化碱降解红树莓籽高聚原花青素及对降血糖酶活性抑制作用

Response Surface Optimization of Alkaline Degradation Process of High Polymeric Proanthocyanidins from Red Raspberry Seed and Inhibitory Activities on Hypoglycemic Enzymes

采用响应面优化碱降解红树莓籽高聚原花青素工艺,并探究高聚原花青素及其水解后的低聚原花青素对降血糖酶活性抑制能力。在单因素基础上,以平均聚合度为响应值,采用Box-Behnken设计优化碱降解工艺,并通过α-Glu和α-Amy抑制率评价高聚原花青素降解前后降血糖效果。结果表明碱液浓度2.13%、料液比1∶10.25 (g/m L)、反应时间42 min、反应温度60℃条件下,原花青素平均聚合度由5.44降为2.14±0.11。红树莓籽高聚原花青素降解前后对α-Glu的IC50分别为0.730、0.291 mg/m L,对α-Amy的IC_(50)分别为0.578、0.342 mg/m L。红树莓籽高聚原花青素降解后提高了降血糖酶活性抑制效果,具有较好的体外降血糖作用,为天然降血糖药物开发提供依据。

The alkaline degradation on red raspberry seed high polymeric proanthocyanidins was optimized by response surface methodology The hypoglycemic enzyme inhibitory ability of high polymeric polyproanthocyanidins and oligomeric procyanidins were evaluated in this research.Based on single-factor test,average polymerization degree of proanthocyanidins was taken as index.Alkaline degradation conditions of high polymeric proanthocyanidins were optimized by Box-Behnken of response surface experiment.And the inhibition effect onα-glucosidase andα-amylase ability of high polymeric polyproanthocyanidins and oligomeric procyanidins were evaluated while inhibition rate was taken as index.The results showed that alkali concentration2.13%,solid-liquid ratio 1∶10.25(mg/m L),Degradation time 42 min,degradation temperature 60℃made the average polymerization degree of proanthocyanidins dropped from 5.44 to 2.14±0.11.The IC50 values ofα-glucosidase for high polymeric polyproanthocyanidins and oligomeric procyanidins were 0.730 and 0.291 mg/m L,and their IC50 forα-amylase were0.578 and 0.342 mg/m L,respectively.The degradation products of high polymeric proanthocyanidins showed a good inhibitory effect onα-glucosidase andα-amylase.And the activity was higher than that of high polymeric polyproanthocyanidins.Thus oligomeric proanthocyanidins have good hypoglycemic activity and are a natural herb with potential hypoglycemic activities.