枸杞玉米黄素双棕榈酸酯脂质体的制备及其体外抗氧化活性

Preparation and in Vitro Antioxidant Activity Determination of Lycium barbarum L. Zeaxanthin Dipalmitate Liposome

为提高枸杞玉米黄素双棕榈酸酯的稳定性和生物利用度,本文采用超声波法制备枸杞玉米黄素双棕榈酸酯脂质体,并通过DPPH清除力、还原力和抗脂质过氧化能力考察其抗氧化活性。结果表明,当玉米黄素双棕榈酸酯,胆固醇,吐温80与卵磷脂的比例分别为1∶6,1∶4和2∶10,以弱酸性磷酸缓冲盐(p H=6.6)为水化介质时,玉米黄素双棕榈酸脂质体的包封率最高,为97.99%。体外抗氧化活性实验结果表明,低包封率的脂质体清除DPPH自由基的能力较小,随着脂质体包封率的增大,其清除DPPH自由基的能力和抗脂质过氧化能力均显著提高(p <0.05),但对于还原力没有显著性影响。本文对玉米黄素双棕榈酸酯脂质体的制备方法及抗氧化活性进行了初探,为其可行性研究提供理论指导。

To improve the stability and bioavailability of Lycium barbarum L. zeaxanthin dipalmitate,the ultrasonic method was used to prepare zeaxanthin dipalmitate liposome. And the antioxidant activity were investigated by scavenging DPPH radical activity,reducing power and the anti-lipid peroxidation activity. The results showed that when the ratio of zeaxanthin dipalmitate,cholesterol and tween 80 with lecithin were 1∶ 6,1∶ 4 and 2∶ 10,respectively,PB buffer with pH6.6,the zeaxanthin dipalmitate liposome whould the highest encapsulation rate,which was 97.99%.The results of antioxidant activity in vitro showed that,liposomes with low envelope sealing rate had less ability to clear DPPH radical,and the DPPH free radical scavenging capacity and anti-lipid peroxidation effect increased significantly( p < 0.05) with the increasing of encapsulation efficiency. But had no significant influence on reducing power. In this paper,the preparation and antioxidation activity in vitro of zeaxanthin dipalmitate liposomes were preliminarily studied,which would provide the theoretical guidance for the feasibility study.