四种小浆果浆汁活性成分及其抗氧化活性

Active Components and Antioxidant Activities of Four Kinds Small Berry Juices

为了对树莓、蓝莓、黑果腺肋花楸及蓝靛果四种小浆果浆汁进行初步活性评价,测定了四种小浆果浆汁的多酚、超氧化物歧化酶(SOD)、花色苷及总黄酮含量,并研究其体外抗氧化活性。结果发现,蓝靛果的多酚(4.659 mg/mL)、SOD(115.38 mg/mL)及总黄酮(10.717 mg/mL)是其他三种小浆果含量的1.5～7倍,花色苷(3.768 mg/mL)则是黑果腺肋花楸含量的14倍左右。以V_C作阳性对照,蓝靛果体外抗氧化效果最优,对·OH、DPPH·自由基的清除及对脂质过氧化的抑制作用的IC_(50)值分别为2.066 mg/mL、61.342μg/mL和181.590μg/mL,对O_2^-·自由基的清除效果并没有明显优势。活性成分含量与抗氧化活性相关性分析表明,四种小浆果浆汁中多酚、花色苷、SOD及总黄酮含量与对·OH、O_2^-·、DPPH·自由基的清除率及对脂质过氧化的抑制率均呈正相关性,且相关性显著。四种小浆果浆汁均具有较好的体外抗氧化活性,蓝靛果显示了最佳的抗氧化活性。

The main purpose of this paper was to evaluate the activities preliminarily of four kinds of small berry such as Rubus corchorifolius L. f.,Vaccinium Spp.,Sorbuspohuashanensis H and Lonicera edulis. The content of polyphenols,superoxide dismutase (SOD),anthocyanins and total flavonoids was determined in the four small berry juices,and their antioxidant activities in vitro were studied.The results showed that the contents of polyphenols (4.659 mg/mL),SOD (115.38 U/mL) and total flavonoids (10.717 mg/mL) in Lonicera edulis were 1.5 ~ 7 times higher than that of the other three small berries,the content of anthocyanin (3.768 mg/mL) was about 14 times higher than that of Sorbuspohuashanensis H.Using VC as the positive control,the Lonicera edulis had the best antioxidation effect in vitro,and the IC50 values for the scavenging of ·OH,DPPH·radicals and lipid peroxidation were 2.066 mg/mL,61.342 μg/mL and 181.590 μg/mL respectively. There was no obvious advantage in the scavenging of O2-·free radical. The correlation analysis showed that the content of polyphenols,anthocyanins,SOD and total flavonoids in the four small berry juices and the clearance rate of·OH,O2-·,DPPH·radicals and the inhibition rate of lipid peroxidation were positively correlated with a significant correlation. The four kinds small berry juices all had good antioxidant activities in vitro,and the Lonicera edulis showed the best antioxidant activities.