高钙菜黄酮的分级分离各级分的抗氧化活性

Antioxidant Activity of Flavonoids Fractionation Extracts from Sedum aizoon L.

以抗坏血酸和叔丁基羟基茴香醚做阳性对照,采用二苯代苦味肼基自由基(DPPH·)、羟自由基(·OH)、超氧阴离子自由基(O_2^-·)、还原力、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐阳离子自由基(ABTS+·)和总抗体外抗氧化模型,主成分分析综合评价高钙菜总黄酮各级分(乙酸乙酯相、正丁醇相、氯仿相和水相)的抗氧化活性。结果显示,在各体外抗氧化活性指标的测定中,各萃取相的抗氧化活性均随着黄酮浓度增加呈现上升趋势,其中ABTS^+·的清除能力最强,高达99.72%±0.10%,与抗坏血酸清除能力相近。乙酸乙酯相的·OH清除率、O_2^-·清除率、总抗氧化FRAP值、还原力及ABTS^+·清除率要显著优于其它各萃取相,氯仿相的DPPH·清除率显著优于其它萃取相。主成分分析综合评价显示,乙酸乙酯相抗氧化活性最佳。显色反应结合红外光谱分析,初步判定乙酸乙酯相和正丁醇相可能为黄酮醇类。高钙菜总黄酮具有较强的抗氧化活性,可作为良好的健康食品原料开发及利用。

The antioxidant model (DPPH radical,hydroxyl radical,superoxide anion radical,reducing power,and ABTS^+ radical) and principal component analysis were used to evaluate the antioxidant abilities of various fractions (normal butyl alcohol,ethyl acetate,chloroform fraction and water) from Sedum aizoon L.using ascorbic acid and hydroxyanisole as control.In the determination of antioxidant activity indicators in vitro,the antioxidant ability of various extraction phases increased with the increase of the concentration of flavonoids. Moreover,the free radical scavenging activities for ABTS^+ radical of them was the strongest,which was equivalent to that of Vit C and reached 99.72% ± 0.10%. The hydroxyl radical,superoxide anion radical and ABTS^+ radical scavenging activity,FRAP value and reducing power of ethyl acetate extract were significantly stronger than those of other solvent extracts.The DPPH radical scavenging rate of normal butyl alcohol extract was higher than those of other solvent extracts.The antioxidant capacity of ethyl acetate extract was strongest according to the principal component analysis results.The results of infrared spectroscopy and color reaction demonstrated the ethyl acetate layer and normal butyl alcohol layer perhaps was flavonols.These data suggested that the flavonoids in Sedum aizoon L.have strong antioxidant activity and can be used as health food raw materials.