基于~1H-NMR代谢组学初步比较真蜂蜜和掺假蜂蜜差异成分

Comparison of Chemical Constituents in Honey and Adulterated Honey Based on ~1H-NMR Metabonomics

采集蜂蜜样品的核磁共振氢图谱(~1H-NMR),通过多元统计分析及相关系数负载图法找出真蜂蜜和掺假蜂蜜的差异性化学成分。结果表明,在蜂蜜的~1H-NMR图谱中,共有24个物质的~1H-NMR信号,包括7个有机酸,15个糖类和2个其他类物质。通过对真蜂蜜和掺假蜂蜜之间的比较分析,得出真蜂蜜组中的苏氨酸、脯氨酸、松二糖、曲二糖、松三糖和苯基丙氨酸含量高于掺假蜂蜜,而2,3-丁二醇、乙偶姻、甘露糖、5-羟甲基糠醛和甲酸的含量低于掺假蜂蜜组,并且都具有显著性差异(p<0.05)。应用NMR代谢组学技术从整体水平上分析蜂蜜样品的化学成分,可以发现真蜂蜜与掺假蜂蜜之间的差异性化学成分,为蜂蜜真假鉴别提供了新思路。

In order to compare the chemical constituents in honey and adulterated honey,the^1H nuclear magnetic resonance(~1H-NMR)and multivariate statistical analysis was used to analyze the chemical constituents of honey and adulterated honey.Twenty four substances were recognized in the^1H-NMR profiling,including seven organic acids,fifteen saccharide and two other substances.It was found that the levels of threonine,proline,turanose,kojibiose,melezitose and phenylalanine were higher,while 2,3-butanediol,acetoin,mannose,5-hydroxymethyl furfural and formic acid were lower in the honey than in adulterated honey.And all these differences were significant(p<0.05).~1H-NMR based metabolomic study on honey could not only be used to identify of adulterated honey,but also analyze the difference components.And it provided the new way and reasonable idea for quality control study of honey.