香芹酚处理对枸杞鲜果采后黑霉病的控制

Control of Carvacrol Treatment on Black Mold of Postharvest Goji Berry

为探究香芹酚处理对宁夏枸杞鲜果采后黑霉病的控制,在离体条件下研究了香芹酚对宁夏枸杞鲜果采后黑霉病菌(Alternaria alternata)生长、菌落形态及其超微结构的影响,并在活体条件下研究了其对枸杞黑霉病的控制效果以及对果实过氧化物酶(POD)和苯丙氨酸解氨酶(PAL)活性的影响。离体试验结果表明:香芹酚处理能显著抑制A.alternata的孢子萌发和菌丝生长,其抑制效果存在浓度依赖效应;与对照相比,浓度为8μL/L香芹酚对菌丝生长和孢子萌发的抑制率分别达到80.81%和57.17%;扫描电镜观察发现香芹酚处理的A.alternata菌丝量少,表面粗糙,粗细不均匀,部分出现塌陷,孢子细胞受到不同程度的破坏。进一步体内试验结果表明:香芹酚处理枸杞果实能抑制损伤接种黑霉病的发病率和病斑扩展速度,并且处理还能提高果实的POD和PAL活性。由此可见,香芹酚可以通过直接治愈和间接诱导抗性来控制枸杞鲜果采后黑霉病,在采后病害控制中具有潜在的应用前景。

This study aimed to investigate the inhibitory effectiveness of carvacrol treatment on the mycelial growth,colony morphology and microstructure of Alternaria alternata and its control effect for Goji berry black mold during the postharvest storage.Furthermore,the effect of carvacrol treatment on peroxidase(POD)and phenylalanine ammonia lyase(PAL)activities in Goji berry fruit were studied.The results showed that carvacrol treatment significantly inhibited the spore germination and mycelial growth of A.alternata and the effectiveness was dose-dependent.Through treated with 8μL/L carvacrol,the inhibition rates of mycelial growth and spore germination reached to 80.81%and 57.17%,respectively.Less mycelial,rough surfaces,uniform thickness and distorted cell were observed after treatment with 8μL/L carvacrol by scanning electron microscopy(SEM).Meanwhile,carvacrol treatment not only resulted in lower disease incidence on inoculated Goji berry with A.alternata,but also enhanced the activities of POD and PAL and prevented disease development.These results suggested that carvacrol,as a safe preservative alternative to synthesis fungicide,might have potential application for postharvest disease control.