摘要
本文以6种代表性甜菊糖苷及其肠道代谢物甜菊醇以及甜菊醇的重排异构体异甜菊醇为对象,研究了上述甜菊化合物的抗氧化性及其对鼠巨噬细胞Raw264.7的细胞毒性和抗炎活性。结果表明,所试甜菊糖苷在100μg/mL内对鼠巨噬细胞的Raw264.7均无细胞毒性,也不会诱导炎症反应;其中异甜菊醇抗炎效果最优,100μg/mL时相对于脂多糖刺激后产生的NO含量降低了约30.7%。所试甜菊糖苷对DPPH·和·OH两种自由基的清除率最高分别为24.8%和13.8%,其对DPPH·的清除能力具有结构相关性,但对·OH清除率均低,无结构相关性;而甜菊酚对DPPH·和ABTS^+·清除率分别为92.1%和29.6%,即甜菊提取物的自由基清除率主要由甜菊酚产生。
In this experiment,the cytotoxicity and anti-inflammatory activity of stevia compounds on mouse macrophage Raw264.7 cells was investigated with 6 representative steviol glycosides along with their intestinal metabolite steviol as well as steviol’s rearrangement product isosteviol.Meanwhile,the antioxidant activity of the above stevia compounds and their cytotoxicity and anti-inflammatory activity against Raw264.7 of murine macrophages were investigated.The results showed that the stevia compounds had no cytotoxicity,nor induced inflammatory reaction in the range of 100μg/mL.Isosteviol showed the best anti-inflammatory effect,when the isosteviol concentration was 100μg/mL,the NO contents produced by stimulation with lipopolysaccharide was reduced about 30.7%.The highest free radical scavenging abilities of the assayed steviol glycosides were 24.8%(DPPH·)and 13.8%(·OH),respectively.There had a structural correlation with the scavenging ability on DPPH free radicals,while there was no structural correlation on hydroxyl free radical clearance.When the experiment was carried out with stevia phenols,the DPPH·and ABTS^+·scavenging rates were 92.1%and 29.6%,respectively.Therefore,the antioxidant activity of stevia extracts could be mainly caused by the stevia phenols.
作者
张童童
隋晓辰
夏咏梅
Khaing zar myint
陈俊名
刘湘
ZHANG Tong-tong;SUI Xiao-chen;XIA Yong-mei;Khaing zar myint;CHEN Jun-ming;LIU Xiang(State Key Laboratory of Food Science and Technology,School of Chemical and Materials Engineering,Jiangnan University,Wuxi 214122,China)
出处
《食品工业科技》
CAS
北大核心
2019年第8期260-265,271,共7页
Science and Technology of Food Industry
基金
国家自然科学基金(31772017)
关键词
甜菊糖苷
甜菊醇
异甜菊醇
抗氧化性
抗炎活性
steviol glycosides
steviol
isosteviol
antioxidant activity
anti-inflammatory activity