硫色镰刀菌(Fusarium sulphureum)体外产毒条件的筛选

Screening of Conditions for Mycotoxin Production by Fusarium sulphureum in vitro

对影响硫色镰刀菌(Fusarium sulphureum)体外产生真菌粗毒素的温度、时间、pH值、培养方式及培养基种类进行筛选。结果表明:F.sulphureum的最佳产毒条件是Richard培养基、25℃、pH 4.5、振荡培养10 d。在单因素筛选的基础上,以温度、pH值和培养时间为自变量,绿豆种子胚根平均长度为响应值,利用响应面分析法对该菌产毒条件的优化结果表明,22.2℃、pH 5.1、振荡培养12 d最有利于体外产毒。

Fusarium sulphureum is a major pathogen causing dry rot of potato tubers, and has a strong ability to produce mycotoxins. The incubation conditions for this pathogenic strain to produce mycotoxin in vitro were screened including temperature, time, pH and stirring condition(static or shaking) and medium type. The results showed that the optimum conditions for mycotoxin production were shaking culture at 25 ℃ for 10 days in Richard liquid medium at pH 4.5. Temperature, pH and culture time were selected by using one-factor-at-a-time designs for further optimization by response surface analysis using the average length of mung bean seed radicles as the response variable. A culture temperature of 22.2 ℃, pH 5.1 and 12 d culture were found optimal for mycotoxin production in vitro.