总状蕨藻多糖体外抗肿瘤作用

Antitumor Activity in vitro of a Polysaccharide from Caulerpa racemosa

以总状蕨藻为原料,采用热水与蛋白酶酶解相结合浸提得到总状蕨藻多糖提取液,经Sevag法和透析法等步骤纯化后得到总状蕨藻粗多糖(Caulerpa racemosa polysaccharide,CRP)。紫外扫描结果表明CRP中含有少量蛋白质。红外光谱分析CRP有一般糖类物质的特征吸收峰,含硫酸基团,是一种酸性多糖。采用噻唑蓝(methylthiazolyldiphenyl-tetrazolium bromide,MTT)法研究了CRP对HeLa、K562、CNE-2z细胞的抑制作用,结果表明CRP对3种肿瘤细胞都有一定的抑制作用,在24~72 h内,随着时间的延长,抑制率逐渐增强,在0.062 5~4 mg/mL质量浓度范围内,随着质量浓度的增加,抑制率逐渐增强,对HeLa、K562和CNE-2z细胞的抑制率最大分别达到61.7%、85.9%和90.3%。

A crude polysaccharide fraction from Caulerpa racemosa(CRP) was obtained through hot water extraction coupled with enzymatic proteolysis, deproteinization by Sevag method and dialysis. Ultraviolet spectroscopy showed CRP to contain a little amount of protein. Infrared spectroscopy revealed that CRP had characteristic polysaccharide absorption peaks and was an acidic polysaccharide containing sulfate groups. The polysaccharide exhibited an antitumor activity against HeLa, K562 and CNE-2z cells, with maximum inhibitory rates of 61.7%, 85.9% and 90.3%, respectively, as determined by MTT assay. This antitumor effect was positively dependent on both exposure time in the range of 24 to 72 h and concentration in the range of 0.062 5 to 4 mg/mL.