内源乳化凝胶化法制备海藻酸钙微胶珠的工艺优化

Optimum Preparation of Calcium Alginate Microbeads by Emulsification/Internal Gelation

通过单因素和正交试验对内源乳化凝胶化法制备海藻酸钙微胶珠的工艺参数进行优化,研究包括海藻酸钠(sodium alginate,NaAlg)质量浓度、Span 80质量浓度、水/油(液体石蜡)两相体积比和CaCO3与NaAlg质量比对海藻酸钙微胶珠粒径分布、外观形态和产率等指标的影响。结果表明:海藻酸钙微胶珠的平均粒径随着NaAlg质量浓度的增大而增大,随着Span 80质量浓度和水油两相体积比值增加而变小,而CaCO3与NaAlg质量比对微胶珠的平均粒径无显著影响。制备粒径在300~600μm,且分布均匀、形态良好、产率较高的海藻酸钙微胶珠的最佳工艺参数为表面活性剂Span 80用量2 g/L、NaAlg质量浓度12 g/L、CaCO3和NaAlg质量比1∶4、水油两相体积比值1∶3。此外,进一步实验表明液体石蜡在重复利用10次后,制得的微胶珠仍保持了良好的形态和得率。

The technological parameters of emulsification/internal gelation for calcium alginate microbeads were optimized by single factor and orthogonal array experiments. The influence of sodium alginate(NaAlg) concentration, Span 80 concentration, volumetric ratio of water to oil(liquid paraffin) and mass ratio of CaCO3 to NaAlg on the morphology, particle size distribution and yield of the microbeads was studied. The results showed that the average particle size of microbeads was increased at higher NaAlg concentrations, but decreased at higher Span 80 concentrations and water to oil ratios, and not significantly affected by the mass ratio of CaCO3 to NaAlg. The optimized conditions for preparing microbeads at 300–600 μmwith good morphology, uniform distribution, and high yield were as follows: 2 g/L Span 80, 12 g/L NaAlg, a mass ratio of CaCO3 to NaAlg of 1:4, and a volumetric ratio of water to oil of 1:3. In addition, further experiments showed that after tenth repeated use of liquid paraffin, microbeads prepared with it remained a good morphology and a higher yield.