超声波协同酶法提取香椿老叶可溶性膳食纤维的工艺优化

Optimization of Ultrasonic-Assisted Enzymatic Hydrolysis for the Extraction of Soluble Dietary Fiber from Old Leaves of Toona sinensis

以香椿老叶为试材,研究超声波协同酶法提取香椿老叶可溶性膳食纤维的影响因素,通过响应面优化试验确定最佳提取工艺参数。结果表明:影响香椿老叶可溶性膳食纤维提取率的因素主次顺序为超声时间>纤维素酶添加量>pH值>超声温度;最佳提取条件为纤维素酶添加量0.50%、超声温度60.0℃、超声时间53.0 min、pH 6.0;此条件下可溶性膳食纤维的提取率可达7.11%,产品杂质含量低,持水力和膨胀力分别为7.29 g/g和4.40 mL/g。

The ultrasonic-assisted enzymatic extraction of soluble dietary fiber(SDF) from old leaves of Toona sinensis was optimized using response surface methodology. Extraction efficiency was affected in decreasing order by ultrasonication time, cellulase dosage, pH and temperature, and the optimum extraction conditions for these four parameters were 53.0 min, 0.50%, 6.0 and 60.0 ℃, respectively. Under the optimized conditions, the extraction yield of SDF was 7.11% with low levels of impurities. The water holding capacity and expansibility of SDF products were 7.29 g/g and 4.40 mL/g, respectively.