高效液相色谱法测定菠萝蜜果实中的葡萄糖、果糖和蔗糖

Determination of Glucose, Fructose and Sucrose in Jackfruit by HPLC

研究菠萝蜜果实葡萄糖、果糖和蔗糖的提取方法并对不同果实部位的糖进行分离,建立能同时测定菠萝蜜果实中3种糖的高效液相色谱方法。CHO-820钙型糖柱为分析柱,柱温90℃,流动相为超纯水,流速0.5 mL/min,进样体积10μL,用示差折光检测器检测。结果表明:100%乙醇是蔗糖的适宜提取液,若同时测定3种糖,则最适提取液为80%乙醇溶液。方法的相对标准偏差为1.51%~7.01%,相关系数在0.999 0以上,加标回收率为98.55%~102.33%;菠萝蜜果肉(以鲜质量计算)中3种糖总含量高达14.01 g/100 g,其中蔗糖含量最高,种皮中糖总含量为10.19 g/100 g,以葡萄糖和果糖为主,果腱中含量最低,仅3.54 g/100 g。不同果实部位果糖与葡萄糖含量相近。

Sugars including glucose, fructose and sucrose in different fruit tissues of jackfruit were extracted by 3 different extraction methods and determined by high performance liquid chromatography(HPLC). The HPLC conditions were set as follows: the chromatograph was equipped with a CHO-820(Ca) column(300 mm × 7.8 mm) and a refractive index detector; ultra-pure water was used as eluent with a flow rate of 0.5 mL/min; the column temperature was 90 ℃. Results showed that 100% ethenol was the optimum extraction solvent for sucrose while 80% ethanol was the best solvent for simultaneous extraction of glucose, fructose and sucrose. The relative standard deviations were in the range from 1.51% to 7.01%, and the recoveries for spiked samples ranged from 98.55% to 102.33% and the correlation coefficients were higher than 0.999 0 for linearity, showing that the method is effective to isolate the three sugars from jackfuit. Results also showed that jackfruit pulp contained 14.01 g/100 g mf sugars on a fresh weight basis with sucrose being the main component while the seed coat contained 10.19 g/100 g mf, with fructose and glucose being the main components and Guojian(developed from unfertilized or partly fertilized female perianth tissues) contained only 3.54 g/100 g mf. The fructose contents were similar to sucrose contents in the 3 different tissues.