太白贝母与暗紫贝母中4种生物碱的含量比较

Comparative Contents of Four Alkaloids in Bulbs of Fritillaria taipaiensis and Fritillaria unibracteata

采用高效液相色谱法对不同商品名称太白贝母与暗紫贝母中贝母辛、西贝母碱苷、贝母素甲、贝母素乙4种生物碱进行测定与分析,为制订质量标准和合理应用提供科学依据。结果表明,由不同品种及同一品种不同商品名称的太白贝母和暗紫贝母中总生物碱提取液的谱图显示,其主要色谱峰基本相似,但相对丰度存在明显差异。太白贝母中贝母辛、西贝母碱苷、贝母素甲和贝母素乙的含量分别为0.153 6~0.231 0、0.060 2~0.140 8、0.029 5~0.044 7、0.006 7~0.019 1 mg/kg;暗紫贝母中贝母辛、西贝母碱苷和贝母素甲含量分别为0.021 5~0.024 1、0.058 7~0.074 4、0.065 8~0.0814 mg/kg,贝母素乙未检出。同时,太白贝母中贝母辛、西贝母碱苷、贝母素甲和贝母素乙成分呈现出随着生长年限(花生大贝、花生小贝>大尖、中尖、小尖>松尖)增加而含量降低的变化趋势,所测太白贝母样品中生物碱的含量略高于暗紫贝母,具有化学等同性。

The contents of four alkaloids, peimisine, sipeimine-3β-D-glucoside, peimine, and peiminine, in bulbs of Fritillaria taipaiensis and Fritillaria unibracteata commercialized with different names were determined by high performance liquid chromatography(HPLC). The results showed that the main chromatographic peaks of alkaloids in all the investigated bulb samples of F. taipaiensis and F. unibracteata were similar but with significant differences in their relative abundances. The contents of peimisine, sipeimine-3β-D-glucoside, peimine, and peiminine were 0.153 6–0.231 0, 0.060 2–0.140 8, 0.029 5–0.044 7 and 0.006 7–0.019 1 mg/kg in Fritillaria taipaiensis bulbs, and 0.021 5–0.024 1, 0.058 7– 0.074 4, 0.065 8–0.0814 mg/kg and at an undetectable level, respectively. All these alkaloids in Fritillaria taipaiensis bulbs exhibited a downward trend with growing age following the decreasing order: Huashengdabei, Huashengxiaobei > Dajian, Zhongjian, Xiaojian > Songjian. The Fritillaria taipaiensis samples investigated in this study contained slightly higher levels of the four alkaloids than the Fritillaria unibracteata samples despite having chemical consistency.