柠檬形克勒克酵母复合CaCl2处理对采后黄花梨果实轮纹病的控制

Biocontrol of Huanghua Pear Ring Rot by Kloeckera apiculata Combined with CaCl_2

研究柠檬形克勒克酵母复合CaCl2处理对采后黄花梨果实轮纹病的控制效果及相关机理。结果表明:采用1×108 CFU/mL柠檬形克勒克酵母结合2%CaCl2处理黄花梨果实对其采后轮纹病有显著的控制效果,且明显优于酵母或CaCl2单独处理。在20℃条件下培养,柠檬形克勒克酵母能在果实伤口处迅速定殖生长,并持续保持较高水平,2%CaCl2对酵母生长状态无显著性影响。同时,柠檬形克勒克酵母与CaCl2结合使用能显著诱导黄花梨果实过氧化物酶、苯丙氨酸解氨酶、几丁质酶、β-1,3-葡聚糖酶活性的增强。研究结果说明了柠檬形克勒克酵母结合2%CaCl2处理能够用于控制黄花梨果实采后轮纹病,诱导果实抗病性是其作用机制之一。

Huanghua pear is highly susceptible to infection by infectious microorganisms during postharvest storage. Pear ring rot, caused by Botryosphaeria berengeriana. f.sp. piricola, is one of the major postharvest diseases. The ability of the yeast Kloeckera apiculata combined with CaCl2 to control ring rot of Huanghua pear fruit during storage and its possible biocontrol mechanism were examined in this study. The results indicated that K. apiculata combined with CaCl2 inhibited pear ring rot more effectively than Kloeckera apiculata or CaCl2 used alone at 20 ℃. K. apiculata around the wounds could multiply quickly at the beginning of incubation and then remain at higher levels, while 2% CaCl2 had no negative impact on its growth. Combined treatment with K. apiculata and 2% CaCl2 caused the accumulation of defense-related enzymes such as polyphenol oxidase(PPO), peroxides(POD), phenylalanine ammonia-lyase(PAL), chitinase(CHI), and β-1, 3-glucanase(GLU) in Huanghua pear fruit. These results indicated that K. apiculata combined with 2% CaCl2 could effectively reduce the incidence of ring rot in Huanghua pear. The induction of resistance is one of the most important mechanisms by which K. apiculata combined with CaCl2 controls pear ring rot.