3种处理方式对葛仙米藻胆蛋白清除超氧阴离子自由基能力的影响

Effect of Pretreatment and Extraction Conditions on Superoxide Anion Radical Scavenging Capacity of Phycobiliprotein from Nostoc sphaeroides Kützing

主要研究反复冻融、溶剂、pH值等对葛仙米藻胆蛋白清除超氧阴离子自由基能力的影响,并探讨藻胆蛋白的模拟胃肠液降解产物对超氧阴离子自由基清除能力的变化情况。结果发现反复冻融不会影响葛仙米藻胆蛋白清除超氧阴离子自由基的能力,磷酸缓冲液可提高藻胆蛋白清除超氧阴离子自由基的能力。pH 5.6和pH 7.3浸提物清除超氧阴离子自由基的能力差异不显著,但pH 8.1浸提液略优于其他两种。随着处理时间延长,模拟肠液消化产物清除超氧阴离子自由基能力逐渐增强,模拟胃液消化产物却逐渐下降。

We explored the effect of pretreatment and extraction conditions(repeated freeze-thawing, extraction solvent and pH) on the superoxide anion radical scavenging capacity of phycobiliprotein from Nostoc sphaeroides Küting. The results revealed that this activity was not affected by repeated freeze-thawing before extraction, but enhanced by using phosphate buffer as the extraction solvent. The extracts at pH 5.6 and pH 7.3 did not show a signifi cant difference from each other with respect to superoxide anion radical scavenging capacity, although both slightly inferior to that obtained at pH 8.1. The superoxide anion radical scavenging capacity of phycobiliprotein from Nostoc sphaeroides Küting became stronger with increasing digestion time in simulated intestinal juice, but was attenuated in simulated gastric juice.