摘要
为研究不同地区蜂胶提取物中组分含量的差异及抗氧化活性的大小,采用75%乙醇溶液对9个蜂胶样品进行提取,以高效液相色谱法(high performance liquid chromatography,HPLC)对不同提取物中多酚类成分进行定性和定量分析。并利用氧化自由基吸收能力(oxygen radical absorbance capacity,ORAC)法和用流式细胞仪Hep G2细胞模型评估了9个蜂胶样品的体外和细胞水平抗氧化能力。结果表明:蜂胶样品中含有包括黄酮、酚酸及酯类物质在内的大量多酚类成分,其中含量较高的黄酮类成分有短叶松素-3-乙酸酯、松属素、柯因、短叶松素、高良姜素,含量较高的酚酸及酯类成分有咖啡酸苯乙酯、p-香豆酸、咖啡酸、异阿魏酸。ORAC法结果显示所有蜂胶样品都具有很好的体外抗氧化能力;在细胞水平上,所有样品也都表现出一定的抗氧化能力。9个总黄酮和总酚酸含量高的蜂胶具有较强的抗氧化能力。
In order to evaluate the polyphenolic composition and antioxidant activity of propolis collected from different areas, nine propolis samples were extracted with 75% aqueous ethanol. The polyphenolic composition of the resulting extracts was analyzed by high performance liquid chromatography-diode array detector(HPLC-DAD). Furthermore, the antioxidant capacity of the extracts was evaluated in vitro by oxygen radical absorbance capacity(ORAC) as well as Hep G2 cellular assays by fl ow cytometry. All the propolis samples were rich in polyphenolics, including fl avonoids, phenolic acids and esters. The fl avonoids mainly included pinobanksin-3-O-acetate, pinocembrin, chrysin, pinobanksin and galangin; the phenolic acids and related esters mainly included caffeic acid phenethyl ester, p-coumaric acid, caffeic acid and isoferulic acid. In addition, the ORAC assay showed that all the propolis samples had free-radical scavenging activities; the same results were obtained in Hep G2 cellular assays. Our results indicated that propolis with strong antioxidant capacity usually contained large amounts of polyphenolic compounds.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第13期59-65,共7页
Food Science
基金
“十二五”国家科技支撑计划项目(2011BAD33B04)
国家现代农业(蜂)产业技术体系建设专项(CARS-45-KXJ18)
关键词
蜂胶
多酚类化合物
抗氧化
propolis
polyphenolics
antioxidant properties
