山楂对果蝇抗氧化相关调控基因表达的影响

Effect of Hawthorn on the Expression of Antioxidant Defense-Related Genes in Drosophila melanogaster

以果蝇为动物模型,研究给予不同剂量山楂提取物(0、0.8、4 mg/mL)对果蝇寿命、抗氧化酶活力及抗氧化相关调控基因表达的影响。结果表明:添加山楂提取物可以延长果蝇寿命;体内抗氧化酶活力测定结果显示,添加0.8 mg/mL山楂提取物可以显著升高铜锌超氧化物歧化酶(CuZn-superoxide dismutase,CuZn-SOD)酶活力(P<0.05),给予4 mg/mL山楂提取物可以极显著升高CuZn-SOD酶活力(P<0.01)和显著升高过氧化氢酶(catalase,CAT)酶活力(P<0.05),且降低丙二醛(malondialdehy de,MDA)水平。实时定量PCR(real timepolymerase chain reaction,real time-PCR),结果显示,给予4 mg/mL山楂提取物显著上调CuZn-SOD、CAT mRNA表达水平(P<0.05);给予0.8 mg/mL山楂提取物磷脂氢谷胱甘肽过氧化物酶(phospholipid hydroperoxide glutathione peroxidase,PHGSH-Px)mRNA表达水平显著升高(P<0.05),4 mg/mL组表达水平极显著升高(P<0.01)。综上,山楂在机体内抗氧化活性可能是通过上调内源性抗氧化酶来实现的。

Hawthorn fruits contain flavonoids, which can play roles in antioxidant capacity. The present research was focused on the effect of dietary supplementation of a hawthorn fruit extract rich in flavonoids at various doses(0, 0.8 and 4 mg/mL) on Drosophila melanogaster life span, antioxidant enzymes activities and related gene expression. The results showed that the life span of Drosophila melanogaster could be prolonged by consumption of the hawthorn fruit extract. Moreover, CuZn-SOD ac tivity was increased significantly(0.8 mg/m L, P < 0.05 and 4 mg/mL, P < 0.01); CAT activity was enhanced significantly at the dose of 4 mg/mL(P < 0.05) while the content of MDA was decreased. Real time-PCR showed that the mRNA expression of CuZn-SOD, CAT(4 mg/mL, P < 0.05), and PHGSH-Px(0.8 mg/mL, P < 0.05; 4 mg/mL, P < 0.01) were increased. Taken together, the hawthorn fruit extract can exert antioxidant activity in vivo via up-regulation of endogenous antioxidant enzymes.