蛹虫草基质多糖对酒精所致小鼠急性肝损伤的保护作用

Protective Effect of Polysaccharides from Cordyceps militaris Stroma against Alcohol-Induced Acute Liver Injury in Mice

目的:研究蛹虫草基质多糖(Cordyceps militaris stroma polysaccharides,CMSP)对酒精所致小鼠急性肝损伤的保护作用。方法:小鼠被随机分为空白对照组、模型组、阳性对照组(150 mg/(kg·d))、CMSP各剂量组(150、300、600 mg/(kg·d)),连续灌胃30 d后,除空白对照组外,给予体积分数50%的乙醇溶液建立小鼠急性肝损伤模型。12 h后,小鼠脱臼处死后取血液测定血浆中超氧化物歧化酶(superoxide dismutase,SOD)活力、甘油三酯(tr i glyceride,TG)含量、谷丙转氨酶(alanine aminotransferase,ALT)和谷草转氨酶(aspartate aminotransferase,AST)活性,取肝脏、脾脏和胸腺计算其系数,并制备肝匀浆测定其中超氧化物歧化酶(superoxide dismutase,SOD)活力、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力、丙二醛(malondialdehyde,MDA)和还原型谷胱甘肽(glutathione transferase,GSH)的含量,并观察肝组织病理学变化。结果:CMSP各剂量组肝脏系数、脾脏系数、胸腺系数,血浆中SOD活力、TG含量、ALT活性、AST活性,肝组织中SOD活力、GSH-Px活力、MDA含量、GSH含量与模型组各水平相比均具有显著性差异(P<0.05或P<0.01)。结论:蛹虫草基质多糖对酒精诱导的小鼠急性肝损伤有明显的保护作用。

Objective: To explore the protective effect of Cordyceps militaris stroma polysaccharides(CMSP) on alcoholinduced acute liver injury in mice. Methods: Mice were randomly divided into blank control, alcohol-induced liver injury model, positive control and CMSP treatment groups(at doses of 150, 300 and 600 mg/(kg·d)) groups. After 30 consecutive days of intragastric a dministration, all mice except for blank control group were given by gavage 50% alcohol at a dose of 12 mL/kg to induce acute liver injury. At 12 h after alcohol administration, all the mice in each group were killed by cervical dislocation and their bloods were collected for the measurement of superoxide dismutase(SOD), alanine transaminase(ALT) and aspartate transaminase(AST) activities and triglyceride(TG) content in the serum. Moreover, the liver, spleen and thymus were collected to calculate the coefficients. The activities of SOD and glutathione peroxidase(GSH-Px) as well as the levels of malondialdehyde(MDA) and glutathione(GSH) in liver homogenate were measured, and pathological changes in liver tissue were observed. Results: The liver, spleen and thymus coefficients, serum SOD, ALT a nd AST activities and TG content, and the contents of MDA and GSH in the liver of mice from the CMSP groups were significantly different from those of the liver injury control group(P < 0.05 or P < 0.01). Conclusion: CMSP has a significant hepatoprotective effect on alcohol-induced acute liver injury in mice.