层层自组装法制备双重修饰脂质体及其体外消化稳定性

Preparation and in vitro Digestive Stability of Double Modified Liposomes through Layer-by-Layer Self-Assembly

以粒径、电位、包覆率、沉淀量为指标,采用层层自组装法制备海藻酸钠(sodium alginate,AL)、壳聚糖(chitosan,CH)双重修饰脂质体海藻酸钠-壳聚糖-脂质体(AL-CH-L),研究AL-CH-L的物化性质、微观形貌及其体外消化稳定性。结果表明:0.6%壳聚糖和0.5%海藻酸钠制备的AL-CH-L平均粒径为(330.6±37.3)nm,分布集中,Zeta电位为(-15.8±0.7)mV,透射电镜观察双重修饰脂质体的外层成功包裹了海藻酸钠和壳聚糖聚合物,呈现典型的核-壳结构。以钙黄绿素为荧光标记物对未修饰和双重修饰脂质体进行体外消化实验,结果表明双重修饰脂质体表现出更高的体外消化稳定性。

Sodium alginate(AL) and chitosan(CH) were coated onto liposome through layer by layer self-assembly technique. The physicochemical and morphological characteristics and in vitro digestive stability of AL-CH-L were observed. The results showed that 0.6% CH and 0.5% AL were the best choice to prepare AL-CH-L. Thus, the AL-CH-L revealed a size of(330.6 ± 37.3) nm with a centralized distribution and a zeta potential of(-15.8 ± 0.7) mV, and a polymer fi lm was successfully coated on the surface of liposome as observed under a transmittance electron microscope(TEM). Meanwhile, an obvious core-shell structure was observed. The in vitro digestion experiment carried out using the fl uorescence marker calcein showed better digestive stability of AL-CH-L when compared with common nanoliposomes.