阿魏侧耳胞外多糖对荷瘤小鼠免疫能力的影响

Effect of Extracellular Polysaccharides Produced by Pleurotus ferulae Lanzi on Immune Function of Tumor-Bearing Mice

通过皮下注射小鼠腹水瘤细胞构建荷瘤小鼠模型,灌胃法给药,采用血液分析仪、细胞培养、酶联免疫吸附等设备和方法测定血液中白细胞和淋巴细胞数量、腹腔巨噬细胞吞噬能力、脾淋巴细胞增殖能力、免疫器官指数、血浆中肿瘤坏死因子-α含量等来研究阿魏侧耳胞外多糖对荷瘤小鼠免疫功能的调节作用。结果显示:与荷瘤对照组相比,灌胃100、200 mg/kg的阿魏侧耳胞外多糖可显著降低荷瘤小鼠瘤体质量(P<0.05)、提高血液中白细胞和淋巴细胞数量(P<0.05)、促进脾淋巴细胞的增殖(P<0.05),增加免疫器官指数(P<0.05)和血浆中肿瘤坏死因子-α的含量(P<0.05);灌胃200 mg/kg的阿魏侧耳胞外多糖可以显著增强荷瘤小鼠腹腔巨噬细胞吞噬能力(P<0.05)。因此,一定浓度的阿魏侧耳胞外多糖可提高荷瘤小鼠的免疫能力。

To investigate the immunoregulatory effect of extracellular polysaccharides produced by Pleurotus ferulae Lanzi immune in tumor-bearing mice, tumor-bearing mice were developed by subcutaneous injection of murine sarcoma S180 and intragastric administration. The numbers of leukocytes and lymphocytes in blood, peritoneal macrophage phagocytic activities, splenic lymphocyte proliferation, immune organ weight indexes and tumor necrosis factor-α(TNF-α) level in blood plasma were examined with an automatic blood analyzer, cell culture in vitro and enzyme-linked immunosorbent assay, respectively. The results showed that extracellular polysaccharides produced by Pleurotus ferulae Lanzi at the concentrations of 100 and 200 mg/kg caused a significant reduction in the weight of tumor(P < 0.05) and a significant increase in the numbers of leukocytes and lymphocytes in blood(P < 0.05), the weight indexes of spleen and thymus gland(P < 0.05) and the concentration of TNF-α in blood plasma(P < 0.05), and also significantly enhanced the proliferation capacity of splenic lymphocytes(P < 0.05); while phagocytic activities of murine peritoneal macrophages were significantly enhanced by the extracellular polysaccharides at the concentration of 200 mg/kg(P < 0.05). Thus, the immune function of tumor-bearing mice could be improved by extracellular polysaccharides produced by Pleurotus ferulae Lanzi at a certain concentration.