柞蚕雄蛾黄酮的提取及其体外抗氧化活性分析

Ultrasound-Assisted Extraction and Antioxidant Activity in vitro of Flavonoids from Male Tussah Moth

研究柞蚕雄蛾黄酮提取工艺及其体外抗氧化作用,采用超声波乙醇提取柞蚕雄蛾黄酮,利用紫外-可见吸收光谱对其进行鉴定,采用正交试验设计优选柞蚕雄蛾黄酮提取的最佳工艺条件。从还原能力以及清除1,1-二苯基苦基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、羟自由基(·OH)、超氧阴离子自由基(O2-·)效果来考察柞蚕雄蛾黄酮的体外抗氧化能力。得到最佳工艺条件为:乙醇体积分数60%、料液比1:40(g/mL)、提取时间45 min、提取温度70℃,在此条件下进行验证实验,柞蚕雄蛾黄酮的一次提取量可达3.72 mg/g。柞蚕雄蛾黄酮对自由基的清除能力较强,均呈现出量效关系,其清除DPPH自由基、·OH、O2-·的IC50值分别为752.4、617.8、813.4μg/mL,其中清除·OH能力要强于VC和2,6-二叔丁基-4-甲基苯酚,清除O2-·能力大于2,6-二叔丁基-4-甲基苯酚。表明柞蚕雄蛾黄酮具有较强的体外抗氧化活性。

Objective: To investigate the extraction and antioxidant effects of flavonoids from male tussah moth. Methods: Flavonoids were ultrasonically extracted from male tussah moth with ethanol. The extract was analyzed qualitatively by UV-visible absorption spectroscopy. The optimum extraction conditions were determined by orthogonal array design. The antioxidant activity of flavonoids from male tussah moth investigated by comparing the reducing power and radical scavenging effects against DPPH, hydroxyl and superoxide anion radicals with those of VC and BHT in vitro. Results: The optimum extraction conditions were as follows: ethanol concentration 60%, solid/solvent ratio 1:40, extraction time 45 min and temperature 70 ℃. Under the optimized conditions, the yield of total flavonoids from male tussah moth was 3.72 mg/g in one extraction cycle. The extracted flavonoids had potent radical scavenging activity in a dose-dependent manner against DPPH, hydroxyl and superoxide anion radicals, and its IC50 was 752.4, 617.8 and 813.4 μg/mL, respectively. The hydroxyl radical scavenging activity was stronger than that of VC and BHT, and the superoxide anion radical activity was stronger than that of BHT. Conclusion: Flavonoids from male tussah moth had strong antioxidant effects in vitro.