软枣猕猴桃根中蒽醌类化合物的分离纯化和含量测定

Isolation, Purification and Detection of Anthraquinone Compounds from the Root of Actinidia arguta Sieb. et Zucc.

利用聚酰胺柱层析和硅胶柱层析法从软枣猕猴桃根中分离纯化蒽醌化合物,采用高效液相色谱法分析法检测软枣猕猴桃根中蒽醌类化合物的含量。结果表明:分离的最佳条件为200目硅胶、φ1.1 cm×100 cm层析柱、洗脱剂流速1.5 mL/min;甲醇-水(90:10,v/v)流动相、254 nm检测波长的高效液相色谱测定软枣猕猴桃根中蒽醌主要组分为大黄素和大黄酚,其含量分别为46 μg/g和157.5 μg/g。

Anthraquinones from the root of Actinidia arguta Sieb. et Zucc. were isolated and purified by polyamide column chromatography and silica gel column chromatography and were determined by high performance liquid chromatography(HPLC). The results showed that the optimum silica gel column chromatographic conditions were selected as 200 mesh silica gel, column dimensions φ 1.1 cm × 100 cm, flow rate 1.5 mL/min and mobile phase composed of a mixture of methanol and water(90:10, v/v). The detection wavelength for anthraquinones was 254 nm. Emodin and chrysophanol as the major anthraquinones were detected in the root of Actinidia arguta Sieb. et Zucc. at levels of 46 and 157.5 μg/g, respectively.