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基于响应曲面法的微囊化保加利亚乳杆菌高密度培养条件优化 被引量:7

Optimization of High Cell Density Culture Conditions for Microencapsulated Lactobacillus bulgaricus by Response Surface Methodology
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摘要 采用响应曲面法对微囊化保加利亚乳杆菌FMG-4高密度培养的培养基组成和培养条件进行优化,结果表明:最佳培养基配方为:在MRS培养基中添加乳清粉97.15 g/L、大豆蛋白粉10.00 g/L、酵母粉7.55 g/L、碳酸钙8.03 g/L、硫酸镁0.30 g/L、硫酸锰0.02 g/L。最优培养条件为:培养温度41.7℃、初始pH 6.9。此时保加利亚乳杆菌FMG-4细胞菌密度可达到3.18×1011 CFU/g。 In this study, the medium composition and culture conditions for microencapsulated Lactobacillus bulgaricusFMG-4 were optimized using response surface methodology. The results showed that the optimal medium for high cell density culture of microencapsulated Lactobacillus bulgaricus FMG-4 was an MRS medium supplemented with 97.15 g/L whey powder, 10.00 g/L soy protein powder, 7.55 g/L yeast powder, 8.03 g/L calcium carbonate, 0.30 g/L magnesium sulfate and 0.02 g/L manganese sulfate. The optimal culture conditions were 41.7 ℃ and an initial pH of 6.9. Under the optimized culture conditions, the density of L. bulgaricus FMG-4 reached 3.18×1011 CFU/g.
出处 《食品科学》 EI CAS CSCD 北大核心 2014年第17期153-159,共7页 Food Science
基金 "十二五"国家科技支撑计划项目(2013BAD18B01-4) 国家高技术研究发展计划(863计划)项目(2011AA100903) 中央高校基本科研业务费专项资金项目(KYZ201419) 国家农业科技成果转化资金项目(2012GB23600639) 江苏高校优势学科建设工程资助项目
关键词 液芯微囊 响应曲面法 保加利亚乳杆菌 高密培养 liquid core microcapsule response surface methodology Lactobacillus bulgaricus high cell density culture
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