摘要
采用响应曲面法对微囊化保加利亚乳杆菌FMG-4高密度培养的培养基组成和培养条件进行优化,结果表明:最佳培养基配方为:在MRS培养基中添加乳清粉97.15 g/L、大豆蛋白粉10.00 g/L、酵母粉7.55 g/L、碳酸钙8.03 g/L、硫酸镁0.30 g/L、硫酸锰0.02 g/L。最优培养条件为:培养温度41.7℃、初始pH 6.9。此时保加利亚乳杆菌FMG-4细胞菌密度可达到3.18×1011 CFU/g。
In this study, the medium composition and culture conditions for microencapsulated Lactobacillus bulgaricusFMG-4 were optimized using response surface methodology. The results showed that the optimal medium for high cell density culture of microencapsulated Lactobacillus bulgaricus FMG-4 was an MRS medium supplemented with 97.15 g/L whey powder, 10.00 g/L soy protein powder, 7.55 g/L yeast powder, 8.03 g/L calcium carbonate, 0.30 g/L magnesium sulfate and 0.02 g/L manganese sulfate. The optimal culture conditions were 41.7 ℃ and an initial pH of 6.9. Under the optimized culture conditions, the density of L. bulgaricus FMG-4 reached 3.18×1011 CFU/g.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第17期153-159,共7页
Food Science
基金
"十二五"国家科技支撑计划项目(2013BAD18B01-4)
国家高技术研究发展计划(863计划)项目(2011AA100903)
中央高校基本科研业务费专项资金项目(KYZ201419)
国家农业科技成果转化资金项目(2012GB23600639)
江苏高校优势学科建设工程资助项目
关键词
液芯微囊
响应曲面法
保加利亚乳杆菌
高密培养
liquid core microcapsule
response surface methodology
Lactobacillus bulgaricus
high cell density culture