摘要
目的:研究多不饱和脂肪酸(polyunsaturated fat acids,PUFA)饮食对大鼠肠道菌群及相关脂肪因子的影响,探讨其作用机理。方法:将30只5周龄健康大鼠分为正常对照组、n-6 PUFA组和n-3 PUFA组,自由摄食饮水8周,每周记录大鼠体质量,实验结束时取大鼠盲肠粪便和肝脏,用实时荧光定量聚合酶链式反应检测双歧杆菌(Bifidobacterium,Bif)、乳酸杆菌(Lactobacillus,Lac)、肠道细菌Akkermansia muciniphila(Akk)和脂肪因子脂肪酸合成酶(fatty acid synthetase,FAS)、禁食诱导脂肪因子(fasting-induced adipose factor,FIAF)的水平;并将大鼠盲肠石蜡包埋、切片、苏木精-伊红染色测量其黏膜厚度。结果:n-3 PUFA组与正常对照组相比,肥胖程度和FAS水平明显降低(P<0.05),Bif、Lac、Akk和FIAF水平明显升高(P<0.05),肠道黏膜厚度明显增加;n-6 PUFA组大鼠的各项指标和正常对照组相比均无统计学差异。结论:n-3 PUFA饮食与n-6 PUFA饮食相比,可改变肠道菌群,抑制肥胖的发生和发展。
Purpose: To determine the effect of PUFA diet on gut microbiota and obesity-related cell factors in rats. Methods: A total of 30 five-week-old healthy female rats were randomly divided into three groups: control group, n-6 PUFAcontaining diet group and n-3 PUFA-containing diet group. The rats had free access to the feeds and water for eight weeks. Body weight was recorded once a week during the trail period and the levels of Bifidobacterium(Bif), Lactobacillus(Lac), Akkermansia muciniphila(Akk) and obesity-related cell factors, fatty acid synthetase(FAS) and fasting-induced adipose factor(FIAF) were evaluated by qRT-PCR at the end of the trial. Results: The adiposity and FAS level in the n-3 PUFAcontaining diet group were significantly lower than that in the control group(P < 0.05), and the levels of Bif, Lac, Akk and FIAF, and the thickness of the intestinal mucosa in the n-3 PUFA-containing diet model group were significantly higher than those in the control group(P < 0.05). Conclusion: An obvious change in gut microbiota was observed in rats fed n-3 PUFAcontaining diet; meanwhile the occurrence and development of adiposity were inhibited.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第17期231-235,共5页
Food Science
基金
山东省自然科学基金项目(ZR2010CM010)
