表面活性剂SDS辅助微波提取金佛手总黄酮工艺及抗氧化活性评价

Microwave Extraction Assisted with the Surfactant Sodium Dodecyl Sulphate and Antioxidant Activity Evaluation of Total Flavonoids from Fingered Citron(Citrus medica var. sarcodactylis)

通过单因素试验和正交试验设计,分别研究了表面活性剂十二烷基磺酸钠(sodium dodecyl sulfonate,SDS)质量分数、料液比、微波功率和微波时间对金佛手总黄酮提取量的影响。确定微波提取最佳工艺条件为表面活性剂SDS质量分数0.6%、料液比1:20(g/mL)、微波功率50 W、微波时间30 s,在此工艺条件下金佛手总黄酮提取量为18.7060 mg/g。金佛手总黄酮对DPPH自由基和ABTS自由基清除率分别为79.15%和65.25%,其抗氧化活性略低于槲皮素。结果表明,金佛手总黄酮具有显著抗氧化活性,可作为抗氧化剂应用到食品和医药领域。

In the present study, the impacts of SDS concentration, material-to-liquid ratio, microwave power, and irradiation time on the extraction of total flavonoids from the fruits of fingered citron(Citrus medica var. sarcodactylis) were explored by single factor and orthogonal array designs. Results indicated that the optimal extraction conditions were determined as follows: SDS concentration, 0.6%; material-to-liquid ratio, 1:20(g/mL); microwave power, 50 W; and irradiation time, 30 s. The yield of total flavonoids under the optimized conditions was 18.7060 mg/g. The total flavonoids scavenged 79.15% of DPPH radical and 65.25% of ABTS radical, showing a slightly weaker radical scavenging activity than quercetin. Therefore, the total flavonoids from fingered citron have potent antioxidant activity and can be used as antioxidants in the food and medicine fields.