啤酒花总黄酮测定方法的比较研究

Comparative Study on Assays for Determination of Flavonoids in Hops(Humulus lupulus L.)

以高效液相色谱法对啤酒花总黄酮含量的测定结果作为评判标准(相对准确值),比较与评价AlCl3分光光度法、Al(NO3)3分光光度法和硼氢化钠/氯醌分光光度法3种比色法测定酒花总黄酮含量的准确性。结果表明,高效液相色谱法测定酒花总黄酮含量为(12.73±0.26)mg/g;AlCl3法测得总黄酮含量为(13.66±0.45)mg/g,与高效液相色谱法偏差率为7.3%;而Al(NO3)3法测定结果为(43.53±1.16)mg/g,与高效液相色谱法的偏差率为241.9%;硼氢化钠/氯醌法测定结果为(70.25±1.42)mg/g,与高效液相色谱法的偏差率为451.8%。提出AlCl3分光光度法比其他2种比色法更适用于酒花总黄酮含量的测定,该方法误差小,准确性好。

The accuracy of three spectrophotometric assays for the determination of flavonoids in hops(Humulus lupulus L.) using respectively AlCl3, Al(NO3)3 and sodium borohydride/chloranil(SBC) was evaluated by comparison with the reference data(relatively more accurate) from HPLC analysis. The results showed tha t the total content of flavonoids in hops was(12.73 ± 0.26) mg/g by HPLC, compared to(13.66 ± 0.45) mg/g,(43.53 ± 1.16) mg/g, and(70.25 ± 1.42) mg/g with a relative deviation of 7.3%, 241.9% and 451.8%, respectively, as spectrophotometrically assayed using AlCl3, Al(NO3)3 and SBC. Therefore, the AlCl3-based assay is proposed to be more suitable for the determination of flavonoids in hops with smaller errors.