ICP-OES法测定密蒙花中多种元素

Determination of Multiple Elements in Flos Buddlejae by Inductively Coupled Plasma Optical Emission Spectrometry

建立电感耦合等离子体发射光谱法测定密蒙花中Na、Mg、P、K、Ca、Mn、Fe、Cu、Zn、Sr人体必需元素和Ni、As、Cd、Pb毒理性元素的分析方法。样品经(硝酸+双氧水)密闭微波消解后用超纯水稀释后进行测定,确定各待测元素的分析波长,通过加入内标元素Y补偿待测元素分析信号的漂移,校正基体效应,改善分析信号的稳定性。在选定的操作条件下,各待测元素的检出限在0.011~25.16 g/L之间,利用标准参考物质(NIST SRM 1515)验证分析方法的准确度和精密度,结果表明,标准物质的分析结果与参考值基本一致,相对标准偏差为2.0%~8.3%。方法简便、快速、准确,能实现密蒙花中多元素的快速分析。

A new method for the determination of essential elements(Na, Mg, P, K, Ca, Mn, Fe, Cu, Zn, and Sr) and toxic elements(Ni, As, Cd, and Pb) in Flos Buddlejae was established using inductively coupled plasma optical emission spectrometry(ICP-OES). Nitric acid and hydrogen peroxide were used jointly to achieve the complete decomposition of the organic matrix in a closed-vessel microwave oven. Appropriate analysis lines for various elements were selected. Y as internal standard element was used to compensate matrix effect and signal drift, and the stability of the instrument was improved. Under the optimal conditions, the detection limits were 0.011–25.16 g/L. The accuracy and precision of this method were confirmed through reference standard materials(NIST SRM 1515, apple leaves). The results showed a good agreement between measured and predicted values for all analyzed elements. The relative standard deviation(RSD) was in the range of 2.0% to 8.3%. This method proved simple, sensitive and precise and could allow simultaneous multi-element determination of Flos Buddlejae.