冷藏兔肉中嗜冷菌的分离鉴定及其产生物胺的特性分析

Identification and Characterization of BiogenicAmine-Producing Psych rophile Bacteria from Refrigerated Rabbit Meat

从冷藏兔肉中分离出10株嗜冷菌,用16S rDNA对菌株进行鉴定。B25为沙雷氏菌(Serratia),其他9株为假单胞菌(Pseudomonas)。采用薄层层析法检测10株菌的产生生物胺能力,检测到B25可产生多种生物胺,其他9株没产生生物胺。将B25菌接到加氨基酸前体的假单胞菌肉汤培养基中培养24h,经高效液相色谱法分析该菌株产生生物胺的种类和含量。最终测得该菌株可产生腐胺、苯乙胺和尸胺,产生这3种生物胺的能力均比较强,测得腐胺的含量为0.41mg/mL,尸胺的含量为0.23mg/mL,苯乙胺的含量为0.05mg/mL。因此,该菌对冷藏兔肉产品的质量安全威胁较大。

Ten psychrotrophic strains were isolated from refrigerated rabbit meat and identified with 16 S rDNA sequencing.B25 was identified as Serratia,and nine other strains were identified as Pseudomonas.The amount of biogenic amines produced by these 10 strains was detected by thin-layer chromatography.B25 strain could produce a variety of biogenic amines,while the other nine strains could not produce any biogenic amine.B25 was inoculated into Pseudomonas broth with amino acid precursors and incubated for 24 hours.The biogenic amines produced by B25 strain were determined by high performance liquid chromatography(HPLC).The results demonstrated that the strain had strong ability to produce putrescine,phenylethylamine and cadaverine which were determined to be 0.41 mg/mL,0.05 mg/mL and 0.23 mg/mL,respectively.Therefore,the strain poses a potential threat to the safety of refrigerated rabbit meat products.