蓝莓多酚对巨噬细胞中iNOS、COX-2基因表达的影响

Effects of Blueberry Polyphenols on the Expression of iNOS and COX-2 Genes in Macrophages

RAW264.7巨噬细胞被脂多糖预刺激后,添加从蓝莓中分离制备的不同质量浓度的可萃取多酚(extractable polyphenols,EPP)和不可萃取多酚(non-extractable polyphenols,NEPP),培养不同的时间,研究不同质量浓度及培养时间对诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)、环氧合酶(cyclooxygenase-2,COX-2)基因相对表达量的影响。结果表明:从蓝莓中分离的EPP和NEPP均能抑制iNOS、COX-2基因表达,在12h培养时间内,2种多酚的抑制效果较好,且EPP的抑制效果明显好于NEPP。这证明蓝莓中的NEPP同EPP一样,可通过抑制iNOS、COX-2mRNA的表达表现出明显的抗炎活性。

The effects of different concentrations of extractable polyphenols(EPP) and non-extractable polyphenols(NEPP) from blueberries on the mRNA levels of inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) in lipopolysaccharides(LPS)-induced RAW264.7 cells at different culture times were studied in this paper.The results showed that both EPP and NEPP from blueberries inhibited the mRNA expression of iNOS and COX-2 genes.During a 12-h incubation period,the inhibitory effects of both EPP and NEPP,especially that of EPP,were significant.It is suggested that EPP and NEPP have significant anti-inflammatory activity by inhibiting the mRNA expression of iNOS and COX-2 genes in macrophages.