摘要
为了解乙烯生物合成调控机理,本研究利用聚合酶链式反应技术,从番茄Micro-Tom中克隆2A11果实特异型启动子和LeHB-1基因,构建LeHB-1正义和反义果实特异表达载体,并导入根癌农杆菌GV3101中,为下一步通过转基因操作来研究转录因子LeHB-1在果实发育过程中的生物学功能奠定了实验基础。
ACO is a key enzyme in the biosynthesis of ethylene.LeHB-1 is the transcription factor of tomato gene ACOl.By studying the activity of transcription factor LeHB-1,one can understand the regulation mechanism of ethylene synthesis.In this study,the sequences of fruit-specific promoter 2A11 and LeHB-1 gene were cloned from tomato by PCR.Then the sense and antisense fruit-specific expression vectors of LeHB-1 were constructed and transformed into Agrobactenum GV3101,and then the plant with the transformation of targeted bacteria was obtained.This research laysafoundationforfurther study of the biological functions of the transcription factor LeHB-1 during the development of fruits via the transformation of tomato.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第19期174-179,共6页
Food Science
基金
国家现代农业(苹果)产业技术体系建设专项(nycytx08-05-02)
