摘要
为了获得效价高、特异性好的甲硝唑（metronidazole,MNZ）的小鼠多抗血清,采用琥珀酸酐法将甲硝唑上的羟基改造成羧基,然后通过碳二亚胺法把改造后的甲硝唑分别与牛血清白蛋白（albumin from bovine serum,BSA）和卵清蛋白（ovalbumin,OVA）进行偶联,制备免疫原MNZ-BSA和包被原MNZ-OVA。经紫外扫描和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳初步鉴定偶联成功。将免疫原MNZ-BSA分成20g/只和50g/只2个剂量分别免疫8周龄的BALB/c小鼠。获得了效价高、特异性好的小鼠多抗血清,效价均达到1:10<sup>4</sup>以上,IC<sub>50</sub>值为61.39ng/mL。
Specific high-titer mouse polyclonal antisera against metronidazole were prepared by converting the hydroxyl groups in the metronidazole molecule into carboxyl groups using succinic anhydride method and conjugating the modified molecule(MNZ) respectively with albumin from bovine serum(BSA) and ovalbumin(OVA) using carbodiimide method to form the immunogen MNZ-BSA and the coating antigen MNZ-OVA.UV spectroscopy and SDS-PAGE confirmed that MNZ was coupled successfully to the carrier protein.Specific high-titer polyclonal antisera were obtained by immunizing Balb/c mice with MNZ-BSA at a dose of 20 D g or 50 D g for each mouse for 8 weeks,respectively,and the titers were achieved to be 1:104 and IC50 was 61.39 ng/mL
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第19期185-188,共4页
Food Science
基金
河南省重大科技专项肉类质量安全检测及溯源技术研究与示范项目(121100110800)
河南省高校科技创新团队支持计划资助项目(13IRTSTHN006)
关键词
甲硝唑
人工抗原
多抗血清
快速检测
metronidazole
artificial antigen
polyclonal antibody
rapid detection
