基于玉米黄浆及淀粉培养的罗耳阿太菌发酵多糖工艺研究

Optimization of Culture Conditions forthe Production of Polysaccharides by Athelia rolfsii in a Medium Consisting of Corn Steep Liquor and Starch

从君子兰(Clivia miniata Regel)上筛选出一株罗耳阿太菌(Athelia rolfsii)AY6657741。通过响应面分析试验确定其较适发酵条件为:培养温度29℃、pH4.55、接种量7%、转速200r/min、发酵培养5.5d;较适培养基成分为:玉米淀粉35g/L、玉米黄浆50mL/L、NaNO_33.1 g/L、KH_2PO_41.0g/L、KCI0.5g/L、MgSO_47H_2O 0.25g/L、柠檬酸1.4g/L,结果表明,在最适条件下菌株粗多糖产量达16.135g/L。

This study was intended to employ response surface methodology to establish the optimum culture conditions and medium components for the production of polysaccharides by Athelia rolfsii AY6657741 isolated from Clivia miniata Regel.\Nhen Atheliarolfsii:AY6657741 was inoculated at an inoculum size of 7%by volume to a culture medium containing35 g/L corn starch,50 mL/L corn steep liquor,3.1 g/L NaNO3,1.0 g/L KH2PO4,0.5 g/L KCI,0.25 g/L MgSO4 ? 7H2 O,and 1.4 g/L citric acid(pH 4.55) and cultivated at 29 °C with a rotation speed of 200 r/min for 5.5 d,16.135 g/L crude polysaccharides were produced.