苦菜茶总黄酮对小鼠酒精性脂肪肝的保护作用

Protective Effect of Total Flavonoid Extracted from Sowthistle Tea on Alcoholic Fatty Liver in Mice

探讨苦菜茶总黄酮对小鼠慢性酒精性脂肪肝的保护作用。采用酒精灌胃小鼠,建立慢性酒精性脂肪肝模型。通过不同剂量苦菜茶总黄酮灌胃12周后,测定小鼠体质量、肝脏质量,血清谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate transaminase,AST)活力,甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)含量,肝脏丙二醛(malondialdehyde,MDA)、谷胱甘肽(glutathione,GSH)含量和超氧化物歧化酶(superoxide dismutase,SOD)活力,以及白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)含量;观察肝脏病理变化。结果表明,与模型组比较,苦菜茶总黄酮剂量组血清ALT、AST活力和TG、TC含量,肝脏MDA、IL-1β、TNF-α含量都不同程度地降低,肝脏SOD活力,GSH含量则有所上升,肝脏肿大有一定程度的减轻,说明苦菜总黄酮对慢性酒精性脂肪肝小鼠的肝脏有一定的保护作用。

The aim of this study was to investigate the protective effect of total flavonoids from sowthistle tea(TFST),the leaves of sowthistle,against chronic alcoholic fatty liver in mice.A mouse model of chronic alcoholic fatty liver was established by intragastric administration of alcohol.Then,the model mice were administered with sowthistle tea extract for 12 weeks.In the present study,body weight,liver weight,the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglyceride(TG),and total cholesterol(TC) in serum and the levels of malondialdehyde(MDA),superoxide dismutase(SOD),glutathione(GSH),interteakin-1 p(IL-1 P),and tumor necrosis factor-a(TNF-a)in liver were determined.Meanwhile,histopathological changes in liver tissues were examined.The results showed that the levels of ALT,AST,TG,TC,MDA,IL-1 p,and TNF-α in liver from the treatment groups were lower than those in the model group,while SOD and GSH activities in liver were higher,and the liver lesions were restored in some degree.It is indicated that sowthistle tea extract has a positive impact on chronic alcoholic fatty liver in mice.