S-8大孔吸附树脂富集紫胶色酸

Enrichment of Laccaic Acid by S-8 Macroporous Resin

以提取液中紫胶色酸的含量为指标,通过静态吸附-解吸和动态吸附-解吸紫胶色酸提取液,确定S-8大孔吸附树脂富集紫胶色酸的工艺参数。结果表明:S-8大孔吸附树脂对紫胶色酸有良好的吸附性能,静态吸附过程中S-8大孔吸附树脂在30℃条件下吸附4.5 h后达到对紫胶色酸的最佳饱和吸附,吸附液流速为2 m L/min时,S-8大孔吸附树脂达到动态饱和最佳吸附;解吸液为95%乙醇溶液、100 m L乙醇中加1.0 m L 2 mol/L的盐酸溶液、解吸液流速3 m L/min时色酸富集效果好,解吸率大于90%;经20次重复吸附/解吸后对紫胶色酸的解吸率依然达到89.50%,树脂可多次重复使用;经大孔吸附树脂富集精制后的紫胶色酸含量由24.77%提高至62.92%,纯度提高了1.54倍,富集后紫胶色酸的得率(以原胶质量计)达到0.52%,说明采用S-8大孔吸附树脂富集紫胶色酸是可行的。

The conditions for static and dynamic adsorption and desorption by S-8 macroporous resin for enriching laccaic acid were investigated by measuring the laccaic acid concentration in crude extract and eluates. The results showed that adsorption capability of S-8 macroporous resin was favorable to laccaic acid. In the static adsorption process, the resin exhibited maximum saturated adsorption at 30 ℃ after 4.5 hours. When the flow rate of the sample solution was 2 m L/min the dynamic adsorption achieved the maximum saturation level. The best enrichment results(more than 90% desorption rate) were obtained when the adsorbed laccaic acid was desorbed using 100 m L of 95% aqueous ethanol with 1.0 m L of 2 mol/Lhydrochloric acid at a flow rate of 3 m L/min. The desorption rate remained as high as 89.50% after twentieth repeated use, suggesting good reusability of the sorbent. After the enrichment process, the purity of laccaic acid was increased from 24.77% to 62.92% with a purification factor of 2.54, and the yield of laccaic acid from lac was 0.52%. This study demonstrates the applicability of S-8 macroporous resin for enriching laccaic acid.