几种醇对Lipozyme RM IM催化卵磷脂乙醇解的影响及其工艺条件优化

Effect of Other Alcohols on Lipozyme RM IM-Catalyzed Ethanolysis of Phosphatidylcholine and Optimization of the Ethanolysis Process

研究脂肪酶Lipozyme RM IM催化大豆卵磷脂(phosphatidylcholine,PC)乙醇解反应制备溶血卵磷脂(lysophosphatidylcholine,LPC)过程中其他几种醇对PC乙醇解的影响,并对乙醇解条件进行了优化。发现正丁醇对PC乙醇解无明显影响,叔丁醇对PC乙醇解有一定的抑制作用,1,2-丙二醇和丙三醇呈现较好的促进作用。最后选用丙三醇作为乙醇解的促进剂,通过响应面分析法确定了无溶剂体系中Lipozyme RM IM催化PC醇解的最佳工艺条件为加酶量15%(以PC质量计,m/m)、反应温度28℃、加水量8%(水/乙醇溶液,V/V)、丙三醇10%(丙三醇/乙醇溶液,V/V)、底物质量浓度1.49 g/m L(PC/乙醇溶液,m/V)、反应时间11 h,此条件下 LPC转化率高达98.2%。实验证明,丙三醇对无溶剂体系中Lipozyme RM IM催化条件下的PC乙醇解反应有很好的促进作用。

The effects of other alcohols on the Lipozyme RM IM-catalyzed ethanolysis of soy phosphatidylcholine(PC) for the preparation of lysophosphatidylcholine(LPC) were studied and the ethanolysis conditions were optimized. The results showed that n-butanol had no significant synergistic or inhibitory effect on the ethanolysis of PC, while tert-butanol displayed an inhibitory effect, and 1,2-propylene glycol and glycerol exhibited a significant synergistic effect. Glycerol was chosen as the synergistic agent for PC ethanolysis. The optimal conditions for the ethanolysis catalyzed by Lipozyme RM IM in solvent-free system were obtained by response surface methodology as follows: lipase dosage, 15%(based on the mass of PC); temperature, 28 ℃; ratio of water to ethanol solution, 8%(V/V); ratio of glycerol to ethanol solution, 10%(V/V); substrate concentration, 1.49 g/m L(PC/ethanol solution ratio, m/V), and reaction time, 11 h. Under these conditions, the conversion rate of LPC was 98.2%. Glycerol exhibited a significant synergistic effect on the ethanolysis of PC.