基于电子舌技术检测商业果汁中脂环酸芽孢杆菌

Detection of Alicyclobacillus sp. Spoilage of Commercial Fruit Juice Beverages by Electronic Tongue

采用法国Alpha M.O.S.公司生产的α-AstreeⅡ型电子舌对7种市售果汁饮料及其接种脂环酸芽孢杆菌样品进行测定,所得数据应用主成分分析法进行辨别分析。2种含乳类果汁饮料未能被区分开,其他5种样品均可区分。将电子舌可区分的6种果汁饮料接种两株脂环酸芽孢杆菌并在45℃条件下培养30 d,所有接种处理中均可检出脂环酸芽孢杆菌。3种浑浊型饮料的检出量较多,大于103 CFU/m L。3种澄清型饮料的检出量较少,小于103 CFU/m L。应用电子舌技术可将6种接种脂环酸芽孢杆菌DSM 3922的果汁饮料与对照组区分开,4种接种脂环酸芽孢杆菌XC-6的果汁饮料与对照组区分开,2种接种脂环酸芽孢杆菌XC-6的混合果汁饮料和橙汁饮料同对照组未能被区分。

Commercial fruit juice beverages and their samples inoculated with Alicyclobacillus sp. isolates were discriminated by an α-Astree Ⅱ electronic tongue with principal component analysis. Except for two beverages mainly containing dairy components, other samples could be separated in different groups. Six beverages that could be discriminated by E-tongue were inoculated with two Alicyclobacillus sp. isolates and were incubated at 45 ℃ for 30 days. All inoculated samples were detected with colonies on Bacillus acidoterrestris plate, but number of colonies varied in different beverages. Three cloudy samples had a higher colony number over 103 CFU/m L and three clear samples had a lower colony number below 103 CFU/m L. All samples inoculated with Alicyclobacillus sp. DSM 3922 could be distinguished from control by E-tongue method. Excepted for mixed fruit juice and orange juice, other samples with Alicyclobacillus sp. XC-6 inoculation could be grouped from the control by E-tongue method.