葡萄籽原花青素提取物对衰老模型小鼠抗氧化作用

Antioxidant Activity of Grape Seed Proanthocyanidin Extract in Aging Mouse Model

目的:研究葡萄籽原花青素提取物对衰老小鼠心脏、肝、脑和血清抗氧化功能的影响。方法:采用纤维素酶辅助浸提葡萄籽原花青素,经大孔树脂吸附纯化后灌胃由D-半乳糖连续皮下注射诱导的亚急性衰老模型小鼠,以各组小鼠心脏、肝、脑和血清中的总抗氧化能力(total antioxidant capacity,T-AOC)、超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力和丙二醛(malondialdehyde,MDA)含量为指标,全面评价葡萄籽原花青素提取物在小鼠体内的抗氧化能力。结果:与空白对照组相比,模型对照组小鼠心脏、肝、脑和血清中T-AOC、SOD、GSH-Px活力均有不同程度的降低,MDA含量有不同程度的升高,其大多变化差异显著,说明小鼠衰老模型构建成功。与模型对照组相比,葡萄籽原花青素提取物各剂量组能增强模型小鼠心脏、肝、脑和血清中T-AOC、SOD、GSH-Px活力,同时降低MDA值,其中大多变化差异显著,而血清中各值变化差异极显著。结论:葡萄籽原花青素提取物能够显著增强亚急性衰老小鼠的抗氧化能力,具有延缓衰老的作用。

Objective: To study the antioxidant activity of grape seed proanthocyanidin extract(GSPE) in aging mice induced by D-galactose. Methods: GSPE was extracted with cellulase and purified by macroporous resin adsorption. The aging ICR mouse model induced by subcutaneous injection of D-galactose was orally administered with GSPE. The content of malondialdehyde(MDA), the activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) and total antioxidant capacity(T-AOC) in cerebrum, heart, liver and serum of the mice were assessed. Results: D-galactose could obviously reduce T-AOC as well as SOD and GSH-Px activities, and enhance MDA contents in mouse cerebrum, heart, liver and serum. In contrast, GSPE could significantly enhance T-AOC, and SOD and GSH-Px activities, and reduce MDA contents in serum, and cerebrum, heart and liver tissues of the aging mice, especially in serum. Conclusion: GSPE could improve the activities of endogenous antioxidant enzymes in aging mice. Therefore, it has good antioxidant and anti-aging effects.