膳食摄入染料木素对ICR小鼠血脂及肝脏的影响

Effects of Dietary Genistein on Serum Lipid and Liver of ICR Mice

目的:研究自胎儿期开始长期膳食摄入染料木素对ICR雌、雄子代小鼠血脂及肝脏的影响。方法:自ICR母鼠妊娠第1天开始分别以0、5、25、50 mg/(kg·d)(以体质量计,下同)的染料木素灌胃母鼠并在子代小鼠出生后继续对子代小鼠进行相同的处理,分别于子代小鼠出生后第21、42、80天对其分离血清和肝脏,测定血清中甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)含量;采用苏木精-伊红染色对子代小鼠肝脏进行组织形态观察。结果:自胎儿期开始长期膳食摄入染料木素可降低第21、42、80天ICR雌、雄子代小鼠的血清TC水平,且这种作用在第42、80天时更显著;自胎儿期开始长期膳食摄入染料木素还可以降低子代小鼠血清中TG和LDL-C水平,且对雄性子代小鼠LDL-C的降低效果更显著。染色结果显示自胎儿期开始长期膳食摄入染料木素可能会增加子代小鼠肝脏脂肪变性的风险。结论:自胎儿期开始长期膳食摄入染料木素可降低子代小鼠血清中TC、TG、LDL-C水平,同时可能会增加子代小鼠肝脏脂肪变性的风险。

In order to study the effects of long-term dietary intake of genistein which starts from maternal mice on the blood lipids and liver of female and male offspring, maternal mice were orally adminstered with 0, 5, 25 and 50 mg/(kg·d) of genistein, and the administrations were conducted on the offspring instead of the mother mice after birth. On the 21 st, 42 ndand 80th days after birth, the contents of serum triglycerides(TG), total cholesterol(TC), low-density lipoprotein cholesterol(LDL-C) of the female and male offspring were detected, and liver morphology were observed by hematoxylin-eosin(HE) stained section. The results showed that long-term dietary genistein intake significantly reduced the serum levels of TC, TG and LDL-C of the offspring mice and serum LDL-C was decreased more significantly in the male offspring. HE staining showed that liver steatosis could be more serious and potentially be developed into cirrhosis in response to genistein intake.