不同品种苹果籽总酚含量与抗氧化相关性研究

Correlation between Total Phenolic Content and Antioxidant Activity in Apple Seeds from Seven Cultivars

为探讨苹果籽总酚含量与抗氧化相关性及其抗氧化的物质基础,以7个品种苹果籽为材料,采用FolinCiocalteu法测定总酚含量,FRAP法、ABTS+·法、O2-·清除法、·OH清除法和DPPH自由基清除法评价抗氧化活性,高效液相色谱法进行多酚成分分析。结果表明:7个品种苹果籽总酚含量范围为5.74~17.44 mg GAE/g(以干质量计),抗氧化性优于茶多酚、VC和2,6-二叔丁基-4-甲基苯酚(2,6-di-tert-butyl-4-methylphenol,BHT),蜜脆苹果籽具有最高的总酚含量和最好的抗氧化能力。除DPPH自由基清除法外,多酚含量与其他4种方法测得的抗氧化数据具有显著正相关(r=0.771~0.984)。根皮苷为苹果籽多酚抗氧化的主要成分。

This study aimed to investigate the correlation between the total phenolic content and antioxidant activity in apple seeds from seven cultivars. The total phenolic content was determined by Folin-Ciocalteu method, the antioxidant activity was evaluated by total reducing capacity, 3-ehtylbenzothiazolin-6-sulfnic acid diammonium salt(ABTS) scavenging, superoxide anion scavenging, hydroxyl free radical scavenging and 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging assays, and the polyphenol composition of apple seeds was analyzed by high performance liquid chromatography(HPLC). It was shown that Honeycrisp exhibited the highest total phenolic content and the best antioxidant properties among seven apple cultivars. Antioxidant activity of polyphenols in apple seeds was significantly positively correlated with total phenolic content(r = 0.771–0.984), with the exception of DPPH scavenging capacity. Compared to tea polyphenols, vitamin C(VC) and 2,6-di-tert-butyl-4-methylphenol(BHT), apple seed polyphenols exhibited better antioxidant activity. Phloridzin, which accounted for 79.76%-86.67% of the total phenolic content as determined by HPLC, was the dominant antioxidant component in apple seeds. Polyphenols extracted from apple seeds could have a wide application prospect because they act as the safe, non-toxic natural antioxidants.