矢车菊素-3-葡萄糖苷对人巨核细胞株Dami增殖分化的影响

Effect of Cyanidin-3-O-β-Glucoside on the Proliferation and Differentiation of Human Megakaryocytic Cell Line Dami

目的:研究植物花色苷单体矢车菊素-3-葡萄糖苷(cyanidin-3-O-β-glucoside,Cy-3-g)对人巨核细胞株Dami的增殖、分化作用。方法:不同浓度的花色苷Cy-3-g(0.05、0.50、5.00、50.00、100.00 mmol/L)与人巨核细胞株Dami细胞共同培养,并将用完全培养基培养的细胞设为对照组。培养结束后,用台盼蓝染色法检测巨核细胞活性、噻唑蓝(methyl thiazolyl tetrazolium,MTT)染色法检测细胞增殖能力,软琼脂细胞集落培养法观察巨核细胞集落生成情况。采用Giemsa染色法观察细胞形态、流式细胞术检测巨核细胞DNA多倍体的生成及细胞表面CD41阳性表达率。结果:与对照组相比,50.00、100.00 mmol/L的Cy-3-g可增强Dami细胞活性,差异具有统计学意义(P<0.05);各Cy-3-g剂量组与对照组相比,均可明显增强Dami细胞增殖能力(P<0.01);Dami细胞集落数目、DNA多倍体数目以及CD41的阳性表达率在50.00、100.00 mmol/L Cy-3-g作用下明显升高,且与对照组相比均具有统计学差异(P<0.01)。结论:Cy-3-g能够明显促进人巨核细胞株Dami的增殖、分化,这可以为花色苷促进血小板生成提供可能的理论依据。

Purpose: To investigate the effects of cyanidin-3-O-β-glucoside(Cy-3-g) on the proliferation and differentiation of human megakaryocytes cell line(Dami). Methods: Megakaryocytes were cultured with or without different concentrations of Cy-3-g(0.05, 0.50, 5.00, 50.00 and 100.00 mmol/L). Cell viability and proliferation were respectively measured by typan blue staining and MTT test. Colony formation of megakaryocytes in soft agar medium from different groups was assayed. After culturing the cells from each group, giemsa staining was used to observe cell morphology. Polyploidy of megakaryocyte DNA and CD41 expression rate were tested by flow cytometry. Results: Cy-3-g at concentrations of 50.00 and 100.00 mmol/L effectively enhanced the viability of Dami cells when compared with the control group(P < 0.05). Dami cells treated with Cy-3-g showed higher cell proliferation than the cells from the control group(P < 0.01). Cy-3-g at 50.00 and 100.00 mmol/L significantly increased the number of colony formation, DNA polyploidy and CD41 expression rate in Dami cells compared to the control group(all P < 0.01). Conclusion: Cy-3-g can promote the proliferation and differentiation of human megakaryocytes, which provides a potential theoretical basis for platelet production induced by anthocyanins.