芦苇叶黄酮类提取物体内体外抗氧化性研究

Antioxidant Properties of Flavonoids Extracted from Leaves of Phragmites communis Trin. in vivo and in vitro

研究醇提芦苇叶黄酮类提取物的体内体外抗氧化性。通过测定芦苇叶黄酮类提取物对体外大鼠肝组织匀浆、肝线粒体丙二醛(malondialdehyde,MDA)生成量及大鼠红细胞溶血的影响。结果表明:芦苇叶黄酮类提取物在0.5~10 mg/m L范围内能抑制大鼠肝组织匀浆及肝线粒体MDA的生成,抑制大鼠红细胞溶血,具有量效关系,表明芦苇叶黄酮类提取物具有体外抗氧化效果。随着剂量的增加,芦苇叶黄酮类提取物可提高S180荷瘤小鼠血清中谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)和超氧化物歧化酶(superoxide dismutase,SOD)活性,降低MDA含量,从而提高其体内抗氧化活性,当剂量达到300 mg/(kg·d)时,芦苇叶黄酮类提取物与10 m L/(kg·d)的氟尿嘧啶均可促进荷瘤小鼠体内抗氧化能力的提高,且二者间没有显著性差异。

The in vivo and in vitro antioxidant properties of flavonoids extracted with ethanol from the dry leaves of Phragmites communis Trin.(PCF) were studied by measuring the effects on hemolysis of rat red blood cells(RBCs) and MDA generation in liver homogenate and mitochondria in vitro. Results indicated that PCF in the concentration range of 0.5–10 mg/m L could inhibit the hemolysis of rat RBCs and the generation of MDA in rat liver homogenate and mitochondria in a dose-dependent manner, suggesting obvious antioxidant activity in vitro. Increased concentrations of PCF could enhance the activities of GSH-Px and SOD and reduce MDA levels in the serum of S180 tumor-bearing mice so as to have elevated antioxidant activity. PCF at the dose of 300 mg/(kg·d) had antioxidant activity comparable to that of 5-FU at 10 m L/(kg·d)for tumor-bearing mice in vivo.