雅安藏茶和低聚木糖复配物润肠通便作用

Individual and Combined Laxative Effects of Ya'an Tibetan Tea and Xylooligosaccharide in Constipation Model Rats

通过建立便秘大鼠模型,研究雅安藏茶、低聚木糖及二者的复配物对便秘模型大鼠的润肠通便作用。取体质量相近、同批次的健康雄性Wister大鼠50只,随机选取5只作为正常对照组,其余大鼠用盐酸洛哌丁胺制造大鼠便秘模型。取造模成功的40只大鼠随机分成8组:模型对照组、雅安藏茶浸液组、低聚木糖组、雅安藏茶与低聚木糖复配低、中、高3个剂量组、阳性对照组、藏茶粉末组,连续7 d灌胃受试样品,测定各组大鼠的采食量、饮水量、体质量增量、最后24 h的粪便粒数、粪便含水率、小肠炭末推进率和肠道菌群等肠道功能评价指标。结果表明,雅安藏茶、低聚木糖及二者的复配物均能够增加大鼠排便次数和质量,软化粪便;促进便秘大鼠小肠的蠕动,促进肠道内有益菌的增殖,抑制有害细菌的生长,且雅安藏茶与低聚木糖具有协同通便效果,其通便效果与复配物质量浓度有关,总体以低聚木糖组与雅安藏茶复配高剂量组效果最好,其效果达到或优于阳性对照物番泻叶,并能使便秘大鼠的排便功能恢复至接近正常对照组。雅安藏茶、低聚木糖及二者的复配物均具有润肠通便的作用且对维持肠道菌群的平衡具有很好的功效。

To evaluate the laxative effect of Ya’an Tibetan tea and/or xylooligosaccharide, the rat constipation model was established. From 50 male Wistar rats, 5 rats with the same age and similar body weights were randomly selected as the normal control group, and the remaining rats were treated with loperamide hydrochloride to establish the rat constipation model. The rats with constipation were randomly divided into 8 groups, including constipation model group, Ya’an Tibetan tea infusion group, xylooligosaccharide group, Ya’an Tibetan tea plus xylooligosaccharide high, medium and low-dose groups, positive control group and Ya’an Tibetan tea powder group. After 7 d administration, feed intake, water intake, body weight gain, fecal pellet number and water content within 24 hours, gastrointestinal transition rate and living bacteria in feces were measured in rats from each group. Results showed that fecal pellet number, body weight and water content were increased, and the number of intestinal enterococci and enterobacteriaceae was reduced, intestinal bifidobacteria and lactobacillia were increased and intestinal transition length were extended in the mice treated with Ya’an Tibetan tea and/or xylooligosaccharide as compared to those from the control group. Ya’an Tibetan tea and xylooligosaccharide had a synergistic laxative effect in a dose-dependent manner, and the best laxative effect, similar or even superior to that of the positive control senna, was observed at the high dose, while restoring the bowel function of constipation rats to nearly the normal level. Ya’an Tibetan tea and xylooligosaccharide mixture have defecating function, and may maintain the balance of the intestinal flora.