牛、羊乳及其乳制品中幽门螺杆菌污染及检测技术研究进展

Recent Progress in Contamination and Detection Techniques of Helicobacter pylori in Milk and Dairy Products

近年来大量研究已经表明幽门螺杆菌(Helicobacter pylori,Hp)可能是种食源性致病菌,而牛、羊乳是其最可能的感染源。食物中较难分离培养出Hp,而聚合酶链式反应相关技术灵敏度高,能检出样品中微量的Hp,可用于检测牛、羊乳中幽门螺杆菌的污染情况,但是与人类临床方面检测Hp技术的多样性、特异性与成熟性相比,食品中Hp快速而有效的检测方法及相关标准还相对缺乏。本文主要就近年来牛、羊乳及其乳制品中幽门螺杆菌污染及检测技术的研究进展进行综述,为进一步完善Hp传播途径与致病机制等研究提供一些参考。

In recent years, a large number of studies have shown that Helicobacter pylori may be a food-borne pathogen,and milk is one of the most likely sources of its infection. Isolation and culture of H. pylori from food samples is exacting and time-consuming. At present, PCR and related technologies, which have high sensitivity, are applied to detect trace amounts of H. pylori in food samples such as milk. However, there is lack of rapid and effective detection methods and related standards for H. pylori in food samples although a variety of specific and mature assays are currently available for clinical use. This review focuses on recent progress in contamination and detection techniques of H. pylori in milk and dairy products, so as to provide some references for further research on transmission and pathogenic mechanisms of H. pylori.