火龙果皮原花青素提取纯化及定性分析

Extraction, Purification and Qualitative Analysis of Proanthocyanidins from Pitaya Peel

采用大孔树脂对原花青素粗品进行分离纯化。比较了AB-8、DM130、ADS-17三种大孔树脂对原花青素的吸附和解吸能力,选择AB-8型大孔树脂为吸附树脂。对AB-8大孔树脂吸附解吸条件进行优化,探讨上样流速、解吸流速和解吸剂体积分数3个因素对分离纯化效果的影响。结果表明:AB-8大孔树脂提纯工艺的最佳条件为上样流速2 m L/min、解吸流速1 m L/min、解吸剂体积分数50%。通过花青素反应与高效液相色谱-质谱对提取物进行定性分析,证实该提取物中含有的原花青素为儿茶素、表儿茶素和二聚体,且原花青素纯度为96.65%。

The crude proanthocyanidins extracted from pitaya peel were purified by macroporous adsorption resin. AB-8 macroporous resin was selected as the best adsorbent for proanthocyanidins based on adsorption and desorption capacities in comparison with DM130 and ADS-17. Effects of sample loading flow rate, elution flow rate and eluent concentration on the purification of pitaya peel proanthocyanidins by AB-8 macroporous resin were investigated. The best results were achieved by sample loading at a flow rate of 2 m L/min followed by elution with 50% ethanol at a flow rate of 1 m L/min. The purified product contained 96.65% proanthocyanidins including catechin, epicatechin and dimmer as indicated by colorimetric observation and qualitative analysis by high performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-ESI-MS).