Ca2+、ATP、ADP和AMP对鸭胸肉中肌动球蛋白解离的影响

Actomyosin Dissociation as Influenced by Ca^(2+), ATP, ADP and AMP

为了解促进肌动球蛋白解离的因素,从鸭胸肉中提取肌动球蛋白,研究Ca^(2+)、三磷酸腺苷(adenosine triphosphate,ATP及其降解产物对肌动球蛋白的解离效果。通过蛋白质免疫印迹技术测定肌动蛋白含量的变化来研究肌动球蛋白的解离情况。研究发现,7.564 mmol/L Ca^(2+)对肌动球蛋白的解离无促进作用(P>0.05),而Ca^(2+)浓度升高到200 mmol/L时,能显著促进肌动球蛋白的解离(P<0.05),单独的ATP对肌动球蛋白的解离无促进作用(P>0.05),Ca^(2+)和ATP共同作用于肌动球蛋白后,可显著促进肌动球蛋白的解离(P<0.05),二磷酸腺苷(adenosine diphosphate,ADP、一磷酸腺苷(adenosine monophosphate,AMP)均可显著促进肌动球蛋白的解离(P<0.05),且不同浓度处理组间无显著性差异(P>0.05)。因此,可以推断ADP、AMP以及Ca^(2+)和ATP的共同作用对肌动球蛋白的解离有促进作用。

To understand the potential factors that promote the dissociation of actomyosin, actomyosin was extracted from duck breast muscle and the effect of Ca^(2+)ATP, and its degradation products on actomyosin dissociation was investigated.The dissociation of actomyosin was evaluated by measuring changes in actin content by Western blotting analysis. Results showed that there was no significant change in actomyosin dissociation when it was treated with 7.5–64 mmol/L Ca^(2+)(P >0.05), whereas actomyosin dissociation was enhanced significantly by 200 mmol/L Ca^(2+)treatment(P < 0.05). ATP treatment alone did not result in a significant change in actomyosin dissociation(P > 0.05), but combinatorial treatment with Ca^(2+)and ATP significantly increased actomyosin dissociation(P < 0.05). Both ADP or AMP remarkably promoted actomyosin dissociation(P < 0.05), but there was no significant difference among different concentration groups(P > 0.05). These results suggest that actomyosin dissociation is significantly improved when the actomyosin is treated with ADP, AMP, or combination of Ca^(2+)and ATP.